Isolation and Identification of Lactic Acid Bacteria from Peranakan Etawa h Crossbred Goat Milk
| Main Authors: | Widodo, Widodo, Indratiningsih, Indratiningsih, Nurliyani, Nurliyani, Endang Wahyuni, Endang Wahyuni, Tiyas Tono Taufiq, Tiyas Tono Taufiq |
|---|---|
| Format: | Proceeding PeerReviewed application/pdf |
| Bahasa: | eng |
| Terbitan: |
, 2014
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/136377/1/Isolation%20and%20Identification%20of%20Lactic%20Acid%20Bacteria%20from%20Peranakan%20Etawa%20h%20Crossbred%20Goat%20Milk.pdf https://repository.ugm.ac.id/136377/ |
| ctrlnum |
136377 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><relation>https://repository.ugm.ac.id/136377/</relation><title>Isolation and Identification of Lactic Acid Bacteria from Peranakan Etawa h Crossbred Goat Milk</title><creator>Widodo, Widodo</creator><creator>Indratiningsih, Indratiningsih</creator><creator>Nurliyani, Nurliyani</creator><creator>Endang Wahyuni, Endang Wahyuni</creator><creator>Tiyas Tono Taufiq, Tiyas Tono Taufiq</creator><subject>Agricultural Land Management</subject><subject>Food technology</subject><subject>Livestock</subject><description>Lactic acid bacteria (LAB) are widely used in dairy fermentation. The aim of this study was to isolate and identify LAB from fresh milk of Peranakan Etawah crossbred goat in Yogyakarta, Indonesia. Fresh milk sample from three different farms were collected after milking and plated in to de Mann Rogosa Sharp e (MRS) agar sup plemented with 0.5% ox bile. A single colony was purified with a streaking method, followed by morphology and 
biochemical identification based on Gram staining, catalase test, motility, spore formation, and their growth on different carbon source s . Molecular identification was conducted based on the sequence of 16S rRNA gene. This study isolated four isolates from Peranakan Etawah 
crossbred goat milk. Based on 16S rRNA gene sequences, isolate M101 and M102 showed homology with Lactococcus garvieae , isolate M103 showed homology with Pediococcus 
pentosaceus , whereas isolate M104 showed homology with Lactobacillus paracasei subsp. Paracasei. Further experiments are being carried out to explore their ability and potential as starter culture for goat milk fermentation. Key Words: Goat milk, Lactic Acid Bacteria, Molecular identification.</description><date>2014-11-10</date><type>Journal:Proceeding</type><type>PeerReview:PeerReviewed</type><type>File:application/pdf</type><language>eng</language><identifier>https://repository.ugm.ac.id/136377/1/Isolation%20and%20Identification%20of%20Lactic%20Acid%20Bacteria%20from%20Peranakan%20Etawa%20h%20Crossbred%20Goat%20Milk.pdf</identifier><identifier> Widodo, Widodo and Indratiningsih, Indratiningsih and Nurliyani, Nurliyani and Endang Wahyuni, Endang Wahyuni and Tiyas Tono Taufiq, Tiyas Tono Taufiq (2014) Isolation and Identification of Lactic Acid Bacteria from Peranakan Etawa h Crossbred Goat Milk. In: The 16th AAAP Congress, 10-14 November 2014, Grha Sabha Pramana Universitas Gadjah Mada. </identifier><recordID>136377</recordID></dc>
|
| language |
eng |
| format |
Journal:Proceeding Journal PeerReview:PeerReviewed PeerReview File:application/pdf File |
| author |
Widodo, Widodo Indratiningsih, Indratiningsih Nurliyani, Nurliyani Endang Wahyuni, Endang Wahyuni Tiyas Tono Taufiq, Tiyas Tono Taufiq |
| title |
Isolation and Identification of Lactic Acid Bacteria from Peranakan Etawa h Crossbred Goat Milk |
| publishDate |
2014 |
| topic |
Agricultural Land Management Food technology Livestock |
| url |
https://repository.ugm.ac.id/136377/1/Isolation%20and%20Identification%20of%20Lactic%20Acid%20Bacteria%20from%20Peranakan%20Etawa%20h%20Crossbred%20Goat%20Milk.pdf https://repository.ugm.ac.id/136377/ |
| contents |
Lactic acid bacteria (LAB) are widely used in dairy fermentation. The aim of this study was to isolate and identify LAB from fresh milk of Peranakan Etawah crossbred goat in Yogyakarta, Indonesia. Fresh milk sample from three different farms were collected after milking and plated in to de Mann Rogosa Sharp e (MRS) agar sup plemented with 0.5% ox bile. A single colony was purified with a streaking method, followed by morphology and
biochemical identification based on Gram staining, catalase test, motility, spore formation, and their growth on different carbon source s . Molecular identification was conducted based on the sequence of 16S rRNA gene. This study isolated four isolates from Peranakan Etawah
crossbred goat milk. Based on 16S rRNA gene sequences, isolate M101 and M102 showed homology with Lactococcus garvieae , isolate M103 showed homology with Pediococcus
pentosaceus , whereas isolate M104 showed homology with Lactobacillus paracasei subsp. Paracasei. Further experiments are being carried out to explore their ability and potential as starter culture for goat milk fermentation. Key Words: Goat milk, Lactic Acid Bacteria, Molecular identification. |
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Universitas Gadjah Mada |
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library:university library |
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Perpustakaan Pusat Universitas Gadjah Mada |
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488 |
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UGM Repository |
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Karya Umum |
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SLEMAN |
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DAERAH ISTIMEWA YOGYAKARTA |
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IOS2744 |
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2016-09-14T18:35:35Z |
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2016-09-22T21:49:43Z |
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dc |
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1765816683088314368 |
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17.538404 |