OPTIMASI MEDIUM PERBANYAKAN IN VITRO TUNAS TALAS KALIURANG (Colocasia esculenta L.) DIPLOID DAN TETRAPLOID
| Main Authors: | Sinaga, Khalisa Aini, Wulandari, Dyah Retno, Ratnadewi, Diah |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)
, 2020
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| Subjects: | |
| Online Access: |
http://ejurnal.bppt.go.id/index.php/JBBI/article/view/3944 http://ejurnal.bppt.go.id/index.php/JBBI/article/view/3944/3854 |
Daftar Isi:
- Taro cv. Kaliurang has a good taste and is tolerant to certain pests and diseases. Its development is hampered by insufficient number of good quality plant materials. This study aimed to obtain an optimum formulation of in vitro shoot-inducing medium of taro cv. Kaliurang. Explants from one diploid and three tretraploid clones were subjected to six treatments of medium formulations with various concentrations of thiamine and adenine in BAP-containing MS media. Shoots were rooted, followed by plantlet acclimatization. The rooting medium was 1⁄2 MS without growth hormones, whereas acclimatization medium was a mixture of sterile soil, husk, and cocopeat. The results showed that MS + 2 mg L-1 BAP + 4 mg L-1 thiamine + 2 mg L-1 adenine was the optimum medium with an average 3.45 shoots per explant. Plantlet acclimatization was successful with 99.1% survival. Flow cytometry confirmed tetraploidy level of the regenerants from 3 tetraploid clones.