2018 Bogor-Jawa Barat Plant Biology Aquilaria malaccensis Biologi Bioteknologi Lingkungan Cinchona alkaloids are in extensive uses Glandular trichome Hyptis capitata Jurnal Biotropia Metroxylon sagu Micropropagation Morphology Stevia rebaudiana tetraploid abiotic stress abscisic acid (ABA) acclimatization adenine benzyl amino purine biomass callus induction carbohydrate cell suspension culture e.g. quinine 11 times more abundant and quinidine 23 fold higher compared to the untreated cells. Particularly the level of quinine which is the most demanded for medical and industrial purposes still need to be improved to approach to or even higher than that of extracted from the conventional source genotipe unggul glikosida steviol idioblast in vitro culture keragaman kultur jaringan media kultur medicinal plants micropropagation morfometri not only for drugs but also for soft drink industries. They are harvested from the bark of trees Cinchona spp. after certain ages and therefore are available over a limited time. Cell culture is an alternative way to continuously produce such secondary metabolites in a much shorter time. Various substances were added in the normal growth media to promote quinoline alkaloids production by cell cultures of Cinchona ledgeriana. At the sixth week of culture or even by precursor tryptophan pemanis alami perakaran phyto-compounds plant growth regulator plantlet growth quinine and cinchonine contents were suppressed by paclobutrazol (PBZ) sago palm stabilitas tetraploid substrat agar substrat pasir the production of quinine and quinidine started to grow whereas the production of cinchonine and cinchonidine tended to decrease. An addition of 5 mg/l PBZ to culture media yielded the highest level of total quinine/quinidine after seven weeks thiamine triterpene vermikulit