Daftar Isi: Molecular and Histochemical Analysis of Jatropha Curcus Linn Trangenic Using Tolerance Antibiotics Hygromycin (hpt) and b-lucuronidase (gus-A) Gane

Main Authors:	Maftuchah, Maftuchah, Zainudin, Agus, Pahlevi, Robby Wahyu, Anggriawan, Arik
Format:	Book PeerReviewed
Bahasa:	eng
Terbitan:	El Sevier , 2015
Subjects:	S Agriculture (General) SB Plant culture
Online Access:	http://eprints.umm.ac.id/56013/24/Peer%20Review%20-%20Maftuchah%20-%20Gus-A%20gene%20histochemicals%20test%20hpt%20gene%20Jatropha%20curcas%20Linn.pdf http://eprints.umm.ac.id/56013/25/Similarity%20-%20Maftuchah%20Zainudin%20Pahlevi%20Anggriawan%20-%20Gus-A%20gene%20histochemicals%20test%20hpt%20gene%20Jatropha%20curcas%20Linn.pdf http://eprints.umm.ac.id/56013/26/Certificate%20-%20Maftuchah%20-%20Gus-A%20gene%20histochemicals%20test%20hpt%20gene%20Jatropha%20curcas%20Linn.pdf http://eprints.umm.ac.id/56013/27/Maftuchah%20Zainudin%20Pahlevi%20Anggriawan%20-%20Gus-A%20gene%20histochemicals%20test%20hpt%20gene%20Jatropha%20curcas%20Linn.pdf http://eprints.umm.ac.id/56013/ https://www.sciencedirect.com/science/article/pii/S1876610215000211

Daftar Isi:

To improve Jatropha curcas’s trait, genetic engineering technology genetic engineering development is needed. This study was aimed to analyze transgenic of J. curcas with plasmid vectors pCambia-1304. The study was conducted using primer hpt (forward) 5’-GATGCCTCCGCTCGAAGTAGCG-3’, (reverse) 5’-GCATCTCCCGCCGTGCAC-3’, gus-A (forward) 5’– GCCATTTGAAGCCGATGTCACGCC-3’ and (reverse) 5’-GTATCGGTGTGAGCGTCGCAGAAC-3’. The PCR results elucidated five plants (16.67 %) positive hpt gene, 22 plants (77.33 %) positive gus-A gene. Histochemical test results showed 16 plants (53.33 %) positive hpt test. A total of 24 plants (80 %) resulted positive gus-A gene on leaf and 11 plants (36.67 %) resulted positive gus-A gene on the root.