Molecular and Histochemical Analysis of Jatropha Curcus Linn Trangenic Using Tolerance Antibiotics Hygromycin (hpt) and b-lucuronidase (gus-A) Gane
| ctrlnum |
56013 |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><relation>http://eprints.umm.ac.id/56013/</relation><title>Molecular and Histochemical Analysis of Jatropha Curcus Linn Trangenic Using Tolerance Antibiotics Hygromycin (hpt) and b-lucuronidase (gus-A) Gane</title><creator>Maftuchah, Maftuchah</creator><creator>Zainudin, Agus</creator><creator>Pahlevi, Robby Wahyu</creator><creator>Anggriawan, Arik</creator><subject>S Agriculture (General)</subject><subject>SB Plant culture</subject><description>To improve Jatropha curcas’s trait, genetic engineering technology genetic engineering development is needed. This study was
aimed to analyze transgenic of J. curcas with plasmid vectors pCambia-1304. The study was conducted using primer hpt
(forward) 5’-GATGCCTCCGCTCGAAGTAGCG-3’, (reverse) 5’-GCATCTCCCGCCGTGCAC-3’, gus-A (forward) 5’–
GCCATTTGAAGCCGATGTCACGCC-3’ and (reverse) 5’-GTATCGGTGTGAGCGTCGCAGAAC-3’. The PCR results
elucidated five plants (16.67 %) positive hpt gene, 22 plants (77.33 %) positive gus-A gene. Histochemical test results showed
16 plants (53.33 %) positive hpt test. A total of 24 plants (80 %) resulted positive gus-A gene on leaf and 11 plants (36.67 %)
resulted positive gus-A gene on the root.</description><publisher>El Sevier</publisher><date>2015</date><type>Book:Book</type><type>PeerReview:PeerReviewed</type><type>Book:Book</type><language>eng</language><identifier>http://eprints.umm.ac.id/56013/24/Peer%20Review%20-%20Maftuchah%20-%20Gus-A%20gene%20histochemicals%20test%20hpt%20gene%20Jatropha%20curcas%20Linn.pdf</identifier><type>Book:Book</type><language>eng</language><identifier>http://eprints.umm.ac.id/56013/25/Similarity%20-%20Maftuchah%20Zainudin%20Pahlevi%20Anggriawan%20-%20Gus-A%20gene%20histochemicals%20test%20hpt%20gene%20Jatropha%20curcas%20Linn.pdf</identifier><type>Book:Book</type><language>eng</language><identifier>http://eprints.umm.ac.id/56013/26/Certificate%20-%20Maftuchah%20-%20Gus-A%20gene%20histochemicals%20test%20hpt%20gene%20Jatropha%20curcas%20Linn.pdf</identifier><type>Book:Book</type><language>eng</language><identifier>http://eprints.umm.ac.id/56013/27/Maftuchah%20Zainudin%20Pahlevi%20Anggriawan%20-%20Gus-A%20gene%20histochemicals%20test%20hpt%20gene%20Jatropha%20curcas%20Linn.pdf</identifier><identifier> Maftuchah, Maftuchah and Zainudin, Agus and Pahlevi, Robby Wahyu and Anggriawan, Arik (2015) Molecular and Histochemical Analysis of Jatropha Curcus Linn Trangenic Using Tolerance Antibiotics Hygromycin (hpt) and b-lucuronidase (gus-A) Gane. In: Energy Procedia. Conference and Exhibition Indonesia - New and Renewable Energy and Energy Conservation (The 3rd Indo EBTKE-ConEx 2014), 65 . El Sevier, pp. 194-202. </identifier><relation>https://www.sciencedirect.com/science/article/pii/S1876610215000211</relation><recordID>56013</recordID></dc>
|
| language |
eng |
| format |
Book:Book Book PeerReview:PeerReviewed PeerReview |
| author |
Maftuchah, Maftuchah Zainudin, Agus Pahlevi, Robby Wahyu Anggriawan, Arik |
| title |
Molecular and Histochemical Analysis of Jatropha Curcus Linn Trangenic Using Tolerance Antibiotics Hygromycin (hpt) and b-lucuronidase (gus-A) Gane |
| publisher |
El Sevier |
| publishDate |
2015 |
| topic |
S Agriculture (General) SB Plant culture |
| url |
http://eprints.umm.ac.id/56013/24/Peer%20Review%20-%20Maftuchah%20-%20Gus-A%20gene%20histochemicals%20test%20hpt%20gene%20Jatropha%20curcas%20Linn.pdf http://eprints.umm.ac.id/56013/25/Similarity%20-%20Maftuchah%20Zainudin%20Pahlevi%20Anggriawan%20-%20Gus-A%20gene%20histochemicals%20test%20hpt%20gene%20Jatropha%20curcas%20Linn.pdf http://eprints.umm.ac.id/56013/26/Certificate%20-%20Maftuchah%20-%20Gus-A%20gene%20histochemicals%20test%20hpt%20gene%20Jatropha%20curcas%20Linn.pdf http://eprints.umm.ac.id/56013/27/Maftuchah%20Zainudin%20Pahlevi%20Anggriawan%20-%20Gus-A%20gene%20histochemicals%20test%20hpt%20gene%20Jatropha%20curcas%20Linn.pdf http://eprints.umm.ac.id/56013/ https://www.sciencedirect.com/science/article/pii/S1876610215000211 |
| contents |
To improve Jatropha curcas’s trait, genetic engineering technology genetic engineering development is needed. This study was
aimed to analyze transgenic of J. curcas with plasmid vectors pCambia-1304. The study was conducted using primer hpt
(forward) 5’-GATGCCTCCGCTCGAAGTAGCG-3’, (reverse) 5’-GCATCTCCCGCCGTGCAC-3’, gus-A (forward) 5’–
GCCATTTGAAGCCGATGTCACGCC-3’ and (reverse) 5’-GTATCGGTGTGAGCGTCGCAGAAC-3’. The PCR results
elucidated five plants (16.67 %) positive hpt gene, 22 plants (77.33 %) positive gus-A gene. Histochemical test results showed
16 plants (53.33 %) positive hpt test. A total of 24 plants (80 %) resulted positive gus-A gene on leaf and 11 plants (36.67 %)
resulted positive gus-A gene on the root. |
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IOS4109.56013 |
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Universitas Muhammadiyah Malang |
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136 |
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library:university library |
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Perpustakaan Universitas Muhammadiyah Malang |
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546 |
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UMM Institutional Repository |
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4109 |
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MALANG |
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JAWA TIMUR |
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IOS4109 |
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2019-11-23T23:30:05Z |
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2019-11-23T23:30:05Z |
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dc |
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1686087608167301120 |
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17.538404 |