OPTIMASI WAKTU KESTABILAN KOMPLEKS DALAM ANALISIS ASPARTAM SECARA SPEKTROFOTOMETRI VISIBEL DAN PENERAPANNYA UNTUK PRODUK GULA DIET
| Main Authors: | , CARLITA YULISTIA, , Prof. Dr. Endang Tri Wahyuni, M.S |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2014
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/131724/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=72227 |
Daftar Isi:
- Validation of aspartame analysis by spectrophotometric UV-Visible method using ninhydrin reagent and the application for dietary sugar have been been carried out. First step of this research was determination the optimum conditions of the analysis, i.e. wavelength and time stability. Determination of the optimum wavelength was done by measuring the absorbances of the complex solution in various wavelengths, and time optimization of complex stability was done by measuring the absorbances of the complex solutions every 30 minutes for 7 hours. The next step was validation the aspartame analysis method by measuring the absorbance of aspartame solutions in various concentration. From these absorbances then the statistics regression was calculated. Five different concentrations of aspartame solutions measured in three replication. Validation parameters for this research were linearity, sensitivity, precision, limit of detection (LOD), and limit of quantification (LOQ). The optimum wavelength of aspartame analysis using ninhydrin reagent was 570 nm and complex from aspartame and ninhydrin reaction reached its maximum amount after 5.5 hours, but for longer time the complex was degraded by hydrolyisis reaction. The result showed that aspartame analysis using spectrophotometric UV-Visible method gave linear correlation at concentration 0-0.019 mole/L and it had high precision and sesitivity. Its detection limit was 2.29x10-3 mole/L and its quantification limit was 7.64x10-3 mole/L. Validated aspartame analysis method has been used to analyze aspartame in dietary sugar sample. The result showed that the concentration of aspartame in solid sample was 226.6 mg/g sample, but aspartame in liquid sample could not be detected.