OPTIMASI WAKTU KESTABILAN KOMPLEKS DALAM ANALISIS ASPARTAM SECARA SPEKTROFOTOMETRI VISIBEL DAN PENERAPANNYA UNTUK PRODUK GULA DIET
| Main Authors: | , CARLITA YULISTIA, , Prof. Dr. Endang Tri Wahyuni, M.S |
|---|---|
| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2014
|
| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/131724/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=72227 |
| ctrlnum |
131724 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><relation>https://repository.ugm.ac.id/131724/</relation><title>OPTIMASI WAKTU KESTABILAN KOMPLEKS DALAM ANALISIS ASPARTAM SECARA SPEKTROFOTOMETRI VISIBEL DAN PENERAPANNYA UNTUK PRODUK GULA DIET</title><creator>, CARLITA YULISTIA</creator><creator>, Prof. Dr. Endang Tri Wahyuni, M.S</creator><subject>ETD</subject><description>Validation of aspartame analysis by spectrophotometric UV-Visible
method using ninhydrin reagent and the application for dietary sugar have been
been carried out. First step of this research was determination the optimum
conditions of the analysis, i.e. wavelength and time stability. Determination of the
optimum wavelength was done by measuring the absorbances of the complex
solution in various wavelengths, and time optimization of complex stability was
done by measuring the absorbances of the complex solutions every 30 minutes for
7 hours. The next step was validation the aspartame analysis method by
measuring the absorbance of aspartame solutions in various concentration. From
these absorbances then the statistics regression was calculated. Five different
concentrations of aspartame solutions measured in three replication. Validation
parameters for this research were linearity, sensitivity, precision, limit of detection
(LOD), and limit of quantification (LOQ). The optimum wavelength of aspartame
analysis using ninhydrin reagent was 570 nm and complex from aspartame and
ninhydrin reaction reached its maximum amount after 5.5 hours, but for longer
time the complex was degraded by hydrolyisis reaction. The result showed that
aspartame analysis using spectrophotometric UV-Visible method gave linear
correlation at concentration 0-0.019 mole/L and it had high precision and
sesitivity. Its detection limit was 2.29x10-3 mole/L and its quantification limit was
7.64x10-3 mole/L. Validated aspartame analysis method has been used to analyze
aspartame in dietary sugar sample. The result showed that the concentration of
aspartame in solid sample was 226.6 mg/g sample, but aspartame in liquid sample
could not be detected.</description><publisher>[Yogyakarta] : Universitas Gadjah Mada</publisher><date>2014</date><type>Thesis:Thesis</type><type>PeerReview:NonPeerReviewed</type><identifier> , CARLITA YULISTIA and , Prof. Dr. Endang Tri Wahyuni, M.S (2014) OPTIMASI WAKTU KESTABILAN KOMPLEKS DALAM ANALISIS ASPARTAM SECARA SPEKTROFOTOMETRI VISIBEL DAN PENERAPANNYA UNTUK PRODUK GULA DIET. UNSPECIFIED thesis, UNSPECIFIED. </identifier><relation>http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=72227</relation><recordID>131724</recordID></dc>
|
| format |
Thesis:Thesis Thesis PeerReview:NonPeerReviewed PeerReview |
| author |
, CARLITA YULISTIA , Prof. Dr. Endang Tri Wahyuni, M.S |
| title |
OPTIMASI WAKTU KESTABILAN KOMPLEKS DALAM ANALISIS ASPARTAM SECARA SPEKTROFOTOMETRI VISIBEL DAN PENERAPANNYA UNTUK PRODUK GULA DIET |
| publisher |
[Yogyakarta] : Universitas Gadjah Mada |
| publishDate |
2014 |
| topic |
ETD |
| url |
https://repository.ugm.ac.id/131724/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=72227 |
| contents |
Validation of aspartame analysis by spectrophotometric UV-Visible
method using ninhydrin reagent and the application for dietary sugar have been
been carried out. First step of this research was determination the optimum
conditions of the analysis, i.e. wavelength and time stability. Determination of the
optimum wavelength was done by measuring the absorbances of the complex
solution in various wavelengths, and time optimization of complex stability was
done by measuring the absorbances of the complex solutions every 30 minutes for
7 hours. The next step was validation the aspartame analysis method by
measuring the absorbance of aspartame solutions in various concentration. From
these absorbances then the statistics regression was calculated. Five different
concentrations of aspartame solutions measured in three replication. Validation
parameters for this research were linearity, sensitivity, precision, limit of detection
(LOD), and limit of quantification (LOQ). The optimum wavelength of aspartame
analysis using ninhydrin reagent was 570 nm and complex from aspartame and
ninhydrin reaction reached its maximum amount after 5.5 hours, but for longer
time the complex was degraded by hydrolyisis reaction. The result showed that
aspartame analysis using spectrophotometric UV-Visible method gave linear
correlation at concentration 0-0.019 mole/L and it had high precision and
sesitivity. Its detection limit was 2.29x10-3 mole/L and its quantification limit was
7.64x10-3 mole/L. Validated aspartame analysis method has been used to analyze
aspartame in dietary sugar sample. The result showed that the concentration of
aspartame in solid sample was 226.6 mg/g sample, but aspartame in liquid sample
could not be detected. |
| id |
IOS2744.131724 |
| institution |
Universitas Gadjah Mada |
| institution_id |
19 |
| institution_type |
library:university library |
| library |
Perpustakaan Pusat Universitas Gadjah Mada |
| library_id |
488 |
| collection |
UGM Repository |
| repository_id |
2744 |
| subject_area |
Karya Umum |
| city |
SLEMAN |
| province |
DAERAH ISTIMEWA YOGYAKARTA |
| repoId |
IOS2744 |
| first_indexed |
2016-09-14T18:34:15Z |
| last_indexed |
2016-09-22T21:48:23Z |
| recordtype |
dc |
| _version_ |
1765816549423185920 |
| score |
17.538404 |