AIRBORNE PENICILLIUM PEMBAWA GEN ALERGEN VACUOLAR SERINE PROTEASE PADA UDARA DALAM RUANGAN RUMAH SAKIT DR. SARDJITO YOGYAKARTA
| Main Authors: | , ANDIKA SIDAR, , Prof. Dr. Ir. Endang Sutriswati Rahayu, MS. |
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| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2013
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| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/126381/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=66600 |
Daftar Isi:
- Airborne mold from indoor environment are vary, which is Penicillium are commonly prevalent. Exsposure of mold dispersed in the air can affect health, such as allergy or hypersensitivity. It is due to mold encoding allergen gene. Vacuolar serine protease gene was reported as the major allergen gene from Penicillium. Existing mold encoding allergen may harm individu inside the room, particularly in the hospital environment. The purpose of this study was to investigate the isolates diversity of indoor airborne mold and identified Penicillium species encoding allergen vacuolar serine protease gene from several indoors air hospital. Methods to accomplish this purpose was started with sampling airborne mold in several rooms using DG-18 medium, then inoculated each isolate into MEA and CYA medium for morphological identification based on macroscopic and microscopic characteristics. Allergen detection was done based on PCR amplification of vacuolar serine protease gene using degenerate primer design and continued with molecular identification of allergenic Penicillium species based on amplification of Î2-tubulin gene. The result showed that total isolates found were 150 isolates which consists of 10 genus, there were Penicillium, Aspergillus, Cladosporium, Eurotium, Curvularia, Alternaria, Paaecilomyces, Fusarium, Chaetomium, and Aureobasidium. Moreover, Penicillium, Aspergillus, and Cladosporium were found predominant. Based on allergen vacuolar serine protease gene detection, Penicillium species encoding vacuolar serine protease allergen gene were P.citrinum (isolated from HIV-Care Room, Cancer Treatment Room, Ear-Nose-Throat Disease Care Room, and Patient Waiting Room), P. steckii (isolated from Emergency Room, Pulmonary-care room, and Ear-Nose-Throat Disease Care Room), and P. copticola (isolated from Polyclinic for Children). Percentage of each Penicillium species encoding allergen gene were 9,3% (P. citrinum), 4,6% (P. steckii), and 3,3% (P. copticola) based on 150 isolates found.