ISOLASI METABOLIT SEKUNDER DARI BUAH, KULIT BATANG DAN DAUN MAHKOTA DEWA (Phaleria macrocarpa (Scheff.) Boerl.) SERTA UJI ANTIOKSIDANNYA
| Main Authors: | , Susilawati, , Prof. Dr. Sabirin Matsjeh |
|---|---|
| Format: | Thesis NonPeerReviewed |
| Terbitan: |
[Yogyakarta] : Universitas Gadjah Mada
, 2013
|
| Subjects: | |
| Online Access: |
https://repository.ugm.ac.id/118829/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=58805 |
| ctrlnum |
118829 |
|---|---|
| fullrecord |
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<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><relation>https://repository.ugm.ac.id/118829/</relation><title>ISOLASI METABOLIT SEKUNDER DARI
BUAH, KULIT BATANG DAN DAUN MAHKOTA DEWA
(Phaleria macrocarpa (Scheff.) Boerl.)
SERTA UJI ANTIOKSIDANNYA</title><creator>, Susilawati</creator><creator>, Prof. Dr. Sabirin Matsjeh</creator><subject>ETD</subject><description>Mahkota dewa is one Indonesian medicinal plants which is used for
treatment of various diseases. The aim of this research is to isolate secondary
metabolites from antioxidant active extract of fruit, bark and leave of mahkota
dewa and to determine the molecular structure of isolated compound using
spectroscopic method and to know the antioxidant activity of the isolated
compound containing phenol group.
Isolation of secondary metabolites from fruit, bark and leave of mahkota
dewa were initiated by extraction using methanol as solvent. After concentrated,
crude methanol extract was extracted using n-hexane, chloroform and ethyl
acetate, respectively. All of extracts, except n-hexane extract were analyzed their
antioxidant activity by DPPH method. Chloroform, ethyl acetate and methanol
extracts from fruit, bark and leave that have antioxidant activity were purified
using column chromatography. The fraction of column chromatography were
purified further by recrystalization and or recolumn chromatography and or TLC
preparative. Structure elucidation was performed by spectroscopy using UV, IR,
1H NMR,13C NMR, HMBC, HMQC, COSY, DEPT and MS. Isolated compound
which contained phenolic group were analyzed their antioxidant activity by DPPH
method. Spectroscopy analysis of phenolic compound with many C chiral atoms
was supported by prediction using a series of computational calculation with
Hyperchem using semi empirical method.
This work resulted 7 isolated compounds i.e. glucoside benzophenone
from ethyl acetate extract of fruit and 6 new compounds. New compounds are
naphtoic acid derivative and isophalerin A from ethyl acetate extract of fruit,
glycoside diterpenoid (phaleriaelide) from methanol extract of fruit,
diepoxylignan (macronone) from ethyl acetate extract of bark, aglycon
benzophenone and isophalerin B from ethyl acetate extract of leave. Antioxidant
activity of the isolated of phenolic compound was benzophenone aglycon (very
strong, IC50 10,57 μg/mL), macronone (weak, IC50 240,14 μg/mL), isophalerin B
(weak, IC50 402,70 μg/mL) and benzophenone glucoside (no have antioxidant
activity). Based on Hyperchem with semiempirical PM3 method, stereoisomer of
macronone is lignan 14 which gave similar result with 13C-NMR data.</description><publisher>[Yogyakarta] : Universitas Gadjah Mada</publisher><date>2013</date><type>Thesis:Thesis</type><type>PeerReview:NonPeerReviewed</type><identifier> , Susilawati and , Prof. Dr. Sabirin Matsjeh (2013) ISOLASI METABOLIT SEKUNDER DARI BUAH, KULIT BATANG DAN DAUN MAHKOTA DEWA (Phaleria macrocarpa (Scheff.) Boerl.) SERTA UJI ANTIOKSIDANNYA. UNSPECIFIED thesis, UNSPECIFIED. </identifier><relation>http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=58805</relation><recordID>118829</recordID></dc>
|
| format |
Thesis:Thesis Thesis PeerReview:NonPeerReviewed PeerReview |
| author |
, Susilawati , Prof. Dr. Sabirin Matsjeh |
| title |
ISOLASI METABOLIT SEKUNDER DARI
BUAH, KULIT BATANG DAN DAUN MAHKOTA DEWA
(Phaleria macrocarpa (Scheff.) Boerl.)
SERTA UJI ANTIOKSIDANNYA |
| publisher |
[Yogyakarta] : Universitas Gadjah Mada |
| publishDate |
2013 |
| topic |
ETD |
| url |
https://repository.ugm.ac.id/118829/ http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=58805 |
| contents |
Mahkota dewa is one Indonesian medicinal plants which is used for
treatment of various diseases. The aim of this research is to isolate secondary
metabolites from antioxidant active extract of fruit, bark and leave of mahkota
dewa and to determine the molecular structure of isolated compound using
spectroscopic method and to know the antioxidant activity of the isolated
compound containing phenol group.
Isolation of secondary metabolites from fruit, bark and leave of mahkota
dewa were initiated by extraction using methanol as solvent. After concentrated,
crude methanol extract was extracted using n-hexane, chloroform and ethyl
acetate, respectively. All of extracts, except n-hexane extract were analyzed their
antioxidant activity by DPPH method. Chloroform, ethyl acetate and methanol
extracts from fruit, bark and leave that have antioxidant activity were purified
using column chromatography. The fraction of column chromatography were
purified further by recrystalization and or recolumn chromatography and or TLC
preparative. Structure elucidation was performed by spectroscopy using UV, IR,
1H NMR,13C NMR, HMBC, HMQC, COSY, DEPT and MS. Isolated compound
which contained phenolic group were analyzed their antioxidant activity by DPPH
method. Spectroscopy analysis of phenolic compound with many C chiral atoms
was supported by prediction using a series of computational calculation with
Hyperchem using semi empirical method.
This work resulted 7 isolated compounds i.e. glucoside benzophenone
from ethyl acetate extract of fruit and 6 new compounds. New compounds are
naphtoic acid derivative and isophalerin A from ethyl acetate extract of fruit,
glycoside diterpenoid (phaleriaelide) from methanol extract of fruit,
diepoxylignan (macronone) from ethyl acetate extract of bark, aglycon
benzophenone and isophalerin B from ethyl acetate extract of leave. Antioxidant
activity of the isolated of phenolic compound was benzophenone aglycon (very
strong, IC50 10,57 Î1⁄4g/mL), macronone (weak, IC50 240,14 Î1⁄4g/mL), isophalerin B
(weak, IC50 402,70 Î1⁄4g/mL) and benzophenone glucoside (no have antioxidant
activity). Based on Hyperchem with semiempirical PM3 method, stereoisomer of
macronone is lignan 14 which gave similar result with 13C-NMR data. |
| id |
IOS2744.118829 |
| institution |
Universitas Gadjah Mada |
| institution_id |
19 |
| institution_type |
library:university library |
| library |
Perpustakaan Pusat Universitas Gadjah Mada |
| library_id |
488 |
| collection |
UGM Repository |
| repository_id |
2744 |
| subject_area |
Karya Umum |
| city |
SLEMAN |
| province |
DAERAH ISTIMEWA YOGYAKARTA |
| repoId |
IOS2744 |
| first_indexed |
2016-09-14T18:30:21Z |
| last_indexed |
2016-09-22T21:44:21Z |
| recordtype |
dc |
| _version_ |
1765816371052019712 |
| score |
17.538404 |