PENENTUAN KINETIKA ENZIM POLIGALAKTURONASE (PG) ENDOGENOUS DARI PULP BIJI KAKAO
| Main Author: | GANDA PUTRA, G.P. |
|---|---|
| Format: | Article application/pdf eJournal |
| Terbitan: |
Jurnal Biologi
, 2014
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| Subjects: | |
| Online Access: |
http://ojs.unud.ac.id/index.php/BIO/article/view/575 |
Daftar Isi:
- This research was conducted to determine the enzyme kinetics (Km and Vmax) of endogenous polygalacturonase (PGs) isolated from cocoa pulp. This research was carried out by assaying PGs activities in various concentration of citrus pectin subtrates which were between 0,1 – 1,0% with 0,1% interval. The result showed that the enzyme kinetics of endogenous PGs from cocoa pulp (Km) was 0,37% and Vmax was 6,69 μmol of galacturonic acid/minute/ml.