Aplikasi Bakteri Asam Laktat dari Inasua sebagai Biopreservatif Ikan Patin (Pangasius sp.) Lactic Acid Bacteria Aplication from Inasua As Biopreservative for Catfish (Pangasius sp.)
| Main Authors: | Dewi, Meilany Ariati, Mubarik, Nisa Rachmania, Desniar, Desniar, Budiarti, Sri |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)
, 2022
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| Subjects: | |
| Online Access: |
http://journal.ipb.ac.id/index.php/jphpi/article/view/39206 http://journal.ipb.ac.id/index.php/jphpi/article/view/39206/23402 |
Daftar Isi:
- Lactic Acid Bacteria or LAB are bacteria that are categorized as generally recognized as safe (GRAS) because they are safe for human health and are non-pathogenic, so they have the potential to be biopreservative. Several metabolic products produced by LAB have antibacterial properties, including bacteriocin, diacetyl, hydrogen peroxide and organic acids. Isolates of L. plantarum IN05 and L. rhamnosus IN13 isolated from fermented inasua food were studied for their potential as biopreservatives because of their antibacterial properties. The content of animal protein in fish is in great demand by the public, one of which is catfish which is in great demand by the public, but catfish is easy to damage and decrease in quality. This is due to the presence of the pathogenic bacterium Listeria monocytogenes which has the ability to grow at cold storage temperatures (refrigeration) to freezing. This study aimed to apply a neutral cell-free supernatant, cell-free supernatant, and bacterial cell biomass from L. rhamnosus IN13 and L. plantarum IN05 isolates and formalin in storage of catfish fillets contaminated with Listeria monocytogenes bacteria. The methods used include the production of antibacterial compounds, determination of the sensitivity of antibacterial compounds to test bacteria, confirmation test of antibacterial compounds with the addition of proteinase K, application of antibacterial compounds to catfish fillets, calculation of total bacterial colonies, bacterial colonies of L. monocytogenes, and lactic acid bacteria, as well as measurement of the pH value of catfish fillet. The SBSN antibacterial compound from L. rhamnosus IN13 has the ability to maintain catfish fillets for up to 14 days of storage in accordance with the requirements for fresh fish that are suitable for consumption according to the 2017 SNI standard on general requirements and guidelines for microbiological testing. The total microbial value was 5.65 log CFU/g, the pH value of catfish fillet meat was 6.37, and the total value of L. monocytogenes bacteria decreased during storage time.
- Bakteri Asam Laktat atau BAL merupakan bakteri yang dikategorikan sebagai generally recognized as safe (GRAS) karena bersifat aman bagi kesehatan manusia dan non patogen sehingga berpotensi sebagai biopreservatif. Beberapa produk metabolisme yang dihasilkan oleh BAL memiliki kemampuan sebagai antibakteri, diantaranya ialah bakteriosin, diasetil, hidrogen peroksida dann asam organik. Isolat L. plantarum IN05 dan L. rhamnosus IN13 yang diisolasi dari makanan fermentasi inasua dikaji potensinya sebagai biopreservatif karena kemampuannya memiliki sebagai antibakteri. Kandungan protein hewani dalam ikan sangat diminati oleh masyarakat, salah satunya adalah ikan patin yang banyak diminati masyarakat, namun ikan patin mudah mengalami kerusakan dan penurunan mutu. Hal ini disebabkan oleh hadirnya bakteri patogen Listeria monocytogenes yang memiliki kemampuan tumbuh pada suhu penyimpanan dingin (refrigerasi) hingga beku. Penelitian ini bertujuan untuk mengaplikasikan supernatan bebas sel netral, supernatan bebas sel, dan biomassa sel bakteri dari isolat L. rhamnosus IN13 dan L. plantarum IN05 serta formalin dalam penyimpanan ikan patin fillet yang terkontaminasi bakteri Listeria monocytogenes. Metode yang digunakan mencakup produksi senyawa antibakteri, penentuan sensitivitas senyawa antibakteri terhadap bakteri uji, uji konfirmasi senyawa antibakteri dengan penambahan proteinase K, aplikasi senyawa antibakteri pada ikan patin fillet, perhitungan total koloni bakteri, koloni bakteri L. monocytogenes, dan bakteri asam laktat, serta pengukuran nilai pH ikan patin fillet. Senyawa antibakteri SBSN dari L. rhamnosus IN13 memiliki kemampuan mempertahankan ikan patin fillet sampai lama penyimpanan 14 hari sesuai dengan syarat ikan segar yang layak untuk dikonsumsi sesuai standar SNI tahun 2017 tentang Persyaratan umum dan pedoman untuk pengujian mikrobiologi. Nilai total mikrob 5,65 log CFU/g, nilai pH daging ikan patin fillet 6,37, dan nilai total bakteri L. monocytogenes yang menurun selama waktu penyimpanan.