Studi biodistribusi resveratrol-peg-asam folat-nanopartikel emas pada tikus sprague dawley betina = Biodistribution study of resveratrol-peg-folic acid-gold nanoparticles conjugates in female sprague dawley rats / Azhoma Gumala
| Main Authors: | Azhoma Gumala, author, Add author: Sutriyo, supervisor, Add author: Fadlina Chany Saputri, supervisor, Add author: Silvia Surini, examiner, Add author: Ari Estuningtyas, examiner, Add author: Herman Suryadi, examiner |
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| Format: | Masters Bachelors |
| Terbitan: |
, 2019
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| Subjects: | |
| Online Access: |
http://lontar.ui.ac.id/detail?id=20501311 |
Daftar Isi:
- <b>ABSTRAK</b><br> Nanopartikel emas telah diteliti untuk sistem penghantaran tertarget obat sitotoksik. Penelitian ini dilakukan untuk mendapatkan hasil karakterisasi dan biodistribusi dari konjugat trans-resveratrol-PEG-Asam Folat-Nanopartikel Emas. Nanopartikel emas disintesis dengan reduksi HAuCl4 menggunakan natrium sitrat. Nanopartikel emas dikonjugasikan dengan PEG-FA dan resveratrol membentuk konjugat resveratrol-PEGAsam Folat-Nanopartikel Emas (rsv-PEG-FA-AuNP). Karakterisasi konjugat rsv-PEG-FAAuNP dilakukan dengan pengukuran partikel, zeta potensial, FTIR, UV, dan TEM. Studi biodistribusi pada tikus Sprague Dawley betina sehat dilakukan setelah 90 menit pemberian injeksi konjugat rsv-PEG-FA-AuNP melalui vena ekor. Hasil karakterisasi rsv-PEG-FAAuNP diperoleh nilai diameter rata-rata nanopartikel dan zeta potensial rsv-PEG-FA-AuNP 249,03 ± 10,31 nm dan -36,33 ± 3,12 mV. Pada uji biodistribusi ditemukan konjugat rsv-PEG-FA-AuNP di ginjal (1,90 ± 0,20 μg/g) dan limfa (2,65 ± 1,18 μg/g) setelah 90 menit pemberian iv, namun resveratrol bebas tidak ditemukan di darah, ginjal, dan limfa setelah 90 menit pemberian iv. Konjugat rsv-PEG-FA-AuNP pada sirkulasi sistemik ditemukan pada waktu yang lebih lama dibandingkan dengan resveratrol bebas dan distribusinya tersebar pada organ otak, ginjal, limpa, hati, dan paru. <hr> <b>ABSTRACT</b><br> Gold nanoparticles had been studied for active targeting purpose of cytotoxic agent. This study was presenting the result of characterization and biodistribution of trans resveratrol-PEG-Folic Acid-Gold Nanoparticle conjugates rsv-PEG-FA-AuNP. Gold nanoparticles were generated by reduction of HAuCl4 using sodium citric. Rsv-PEG-FA-AuNPs were produced by conjugation of gold nanoparticles with PEG-folic acid and resveratrol. Characterization of rsv-PEG-FA-AuNP conjugates were held by examination of particle size, zeta potential, FTIR, and TEM. Biodistribution study in female Sprague-Dawley rats conducted after 90 minutes i.v tail vein delivery of rsv-PEG-FA-AuNP conjugates. The mean particle size and zeta potential of rsv-PEG-FA-AuNP were 249.03 10.31 and -36.33 3.12 respectively. Transmission electron microscopy showed almost spherical shape of rsv-PEG-FA-AuNP conjugates. Rsv-PEG-FA-AuNP conjugates were found in kidney 1.90 0.20 μg/g and spleen 2.65 1.18 μg/g after 90 minutes i.v. delivery in female Sprague-Dawley rats. Resveratrol-PEG-FA-AUNP conjugates have longer systemic circulation than free resveratrol and restrained throughout brain, spleen, kidney, lung, and liver after distribution.