Studi biodistribusi resveratrol-peg-asam folat-nanopartikel emas pada tikus sprague dawley betina = Biodistribution study of resveratrol-peg-folic acid-gold nanoparticles conjugates in female sprague dawley rats / Azhoma Gumala
| Main Authors: | Azhoma Gumala, author, Add author: Sutriyo, supervisor, Add author: Fadlina Chany Saputri, supervisor, Add author: Silvia Surini, examiner, Add author: Ari Estuningtyas, examiner, Add author: Herman Suryadi, examiner |
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| Format: | Masters Bachelors |
| Terbitan: |
, 2019
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| Subjects: | |
| Online Access: |
http://lontar.ui.ac.id/detail?id=20501311 |
| ctrlnum |
20501311 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><type>Thesis:Masters</type><title>Studi biodistribusi resveratrol-peg-asam folat-nanopartikel emas pada tikus sprague dawley betina = Biodistribution study of resveratrol-peg-folic acid-gold nanoparticles conjugates in female sprague dawley rats /
Azhoma Gumala</title><creator>Azhoma Gumala, author</creator><creator>Add author: Sutriyo, supervisor</creator><creator>Add author: Fadlina Chany Saputri, supervisor</creator><creator>Add author: Silvia Surini, examiner</creator><creator>Add author: Ari Estuningtyas, examiner</creator><creator>Add author: Herman Suryadi, examiner</creator><publisher/><date>2019</date><subject>Gold nanoparticles</subject><subject>Sprague Dawley rats</subject><description><b>ABSTRAK</b><br>
Nanopartikel emas telah diteliti untuk sistem penghantaran tertarget obat sitotoksik.
Penelitian ini dilakukan untuk mendapatkan hasil karakterisasi dan biodistribusi dari
konjugat trans-resveratrol-PEG-Asam Folat-Nanopartikel Emas. Nanopartikel emas
disintesis dengan reduksi HAuCl4 menggunakan natrium sitrat. Nanopartikel emas
dikonjugasikan dengan PEG-FA dan resveratrol membentuk konjugat resveratrol-PEGAsam Folat-Nanopartikel Emas (rsv-PEG-FA-AuNP). Karakterisasi konjugat rsv-PEG-FAAuNP dilakukan dengan pengukuran partikel, zeta potensial, FTIR, UV, dan TEM. Studi biodistribusi pada tikus Sprague Dawley betina sehat dilakukan setelah 90 menit pemberian injeksi konjugat rsv-PEG-FA-AuNP melalui vena ekor. Hasil karakterisasi rsv-PEG-FAAuNP diperoleh nilai diameter rata-rata nanopartikel dan zeta potensial rsv-PEG-FA-AuNP 249,03 ± 10,31 nm dan -36,33 ± 3,12 mV. Pada uji biodistribusi ditemukan konjugat rsv-PEG-FA-AuNP di ginjal (1,90 ± 0,20 &#956;g/g) dan limfa (2,65 ± 1,18 &#956;g/g) setelah 90 menit pemberian iv, namun resveratrol bebas tidak ditemukan di darah, ginjal, dan limfa setelah 90 menit pemberian iv. Konjugat rsv-PEG-FA-AuNP pada sirkulasi sistemik ditemukan pada waktu yang lebih lama dibandingkan dengan resveratrol bebas dan distribusinya tersebar pada organ otak, ginjal, limpa, hati, dan paru.
<hr>
<b>ABSTRACT</b><br>
Gold nanoparticles had been studied for active targeting purpose of cytotoxic agent. This study was presenting the result of characterization and biodistribution of trans resveratrol-PEG-Folic Acid-Gold Nanoparticle conjugates rsv-PEG-FA-AuNP. Gold nanoparticles were generated by reduction of HAuCl4 using sodium citric. Rsv-PEG-FA-AuNPs were produced by conjugation of gold nanoparticles with PEG-folic acid and resveratrol.
Characterization of rsv-PEG-FA-AuNP conjugates were held by examination of particle size, zeta potential, FTIR, and TEM. Biodistribution study in female Sprague-Dawley rats conducted after 90 minutes i.v tail vein delivery of rsv-PEG-FA-AuNP conjugates. The mean particle size and zeta potential of rsv-PEG-FA-AuNP were 249.03 10.31 and -36.33
3.12 respectively. Transmission electron microscopy showed almost spherical shape of rsv-PEG-FA-AuNP conjugates. Rsv-PEG-FA-AuNP conjugates were found in kidney 1.90 0.20 &#956;g/g and spleen 2.65 1.18 &#956;g/g after 90 minutes i.v. delivery in female Sprague-Dawley rats. Resveratrol-PEG-FA-AUNP conjugates have longer systemic circulation than free resveratrol and restrained throughout brain, spleen, kidney, lung, and liver after distribution.</description><identifier>http://lontar.ui.ac.id/detail?id=20501311</identifier><recordID>20501311</recordID></dc>
|
| format |
Thesis:Masters Thesis Thesis:Bachelors |
| author |
Azhoma Gumala, author Add author: Sutriyo, supervisor Add author: Fadlina Chany Saputri, supervisor Add author: Silvia Surini, examiner Add author: Ari Estuningtyas, examiner Add author: Herman Suryadi, examiner |
| title |
Studi biodistribusi resveratrol-peg-asam folat-nanopartikel emas pada tikus sprague dawley betina = Biodistribution study of resveratrol-peg-folic acid-gold nanoparticles conjugates in female sprague dawley rats /
Azhoma Gumala |
| publishDate |
2019 |
| topic |
Gold nanoparticles Sprague Dawley rats |
| url |
http://lontar.ui.ac.id/detail?id=20501311 |
| contents |
<b>ABSTRAK</b><br>
Nanopartikel emas telah diteliti untuk sistem penghantaran tertarget obat sitotoksik.
Penelitian ini dilakukan untuk mendapatkan hasil karakterisasi dan biodistribusi dari
konjugat trans-resveratrol-PEG-Asam Folat-Nanopartikel Emas. Nanopartikel emas
disintesis dengan reduksi HAuCl4 menggunakan natrium sitrat. Nanopartikel emas
dikonjugasikan dengan PEG-FA dan resveratrol membentuk konjugat resveratrol-PEGAsam Folat-Nanopartikel Emas (rsv-PEG-FA-AuNP). Karakterisasi konjugat rsv-PEG-FAAuNP dilakukan dengan pengukuran partikel, zeta potensial, FTIR, UV, dan TEM. Studi biodistribusi pada tikus Sprague Dawley betina sehat dilakukan setelah 90 menit pemberian injeksi konjugat rsv-PEG-FA-AuNP melalui vena ekor. Hasil karakterisasi rsv-PEG-FAAuNP diperoleh nilai diameter rata-rata nanopartikel dan zeta potensial rsv-PEG-FA-AuNP 249,03 ± 10,31 nm dan -36,33 ± 3,12 mV. Pada uji biodistribusi ditemukan konjugat rsv-PEG-FA-AuNP di ginjal (1,90 ± 0,20 μg/g) dan limfa (2,65 ± 1,18 μg/g) setelah 90 menit pemberian iv, namun resveratrol bebas tidak ditemukan di darah, ginjal, dan limfa setelah 90 menit pemberian iv. Konjugat rsv-PEG-FA-AuNP pada sirkulasi sistemik ditemukan pada waktu yang lebih lama dibandingkan dengan resveratrol bebas dan distribusinya tersebar pada organ otak, ginjal, limpa, hati, dan paru.
<hr>
<b>ABSTRACT</b><br>
Gold nanoparticles had been studied for active targeting purpose of cytotoxic agent. This study was presenting the result of characterization and biodistribution of trans resveratrol-PEG-Folic Acid-Gold Nanoparticle conjugates rsv-PEG-FA-AuNP. Gold nanoparticles were generated by reduction of HAuCl4 using sodium citric. Rsv-PEG-FA-AuNPs were produced by conjugation of gold nanoparticles with PEG-folic acid and resveratrol.
Characterization of rsv-PEG-FA-AuNP conjugates were held by examination of particle size, zeta potential, FTIR, and TEM. Biodistribution study in female Sprague-Dawley rats conducted after 90 minutes i.v tail vein delivery of rsv-PEG-FA-AuNP conjugates. The mean particle size and zeta potential of rsv-PEG-FA-AuNP were 249.03 10.31 and -36.33
3.12 respectively. Transmission electron microscopy showed almost spherical shape of rsv-PEG-FA-AuNP conjugates. Rsv-PEG-FA-AuNP conjugates were found in kidney 1.90 0.20 μg/g and spleen 2.65 1.18 μg/g after 90 minutes i.v. delivery in female Sprague-Dawley rats. Resveratrol-PEG-FA-AUNP conjugates have longer systemic circulation than free resveratrol and restrained throughout brain, spleen, kidney, lung, and liver after distribution. |
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Universitas Indonesia |
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