Data from: The telomeric DNA damage response occurs in the absence of chromatin decompaction
| Main Authors: | Vancevska, Aleksandra, Douglass, Kyle M., Pfeiffer, Verena, Manley, Suliana, Lingner, Joachim |
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| Format: | info dataset Journal |
| Terbitan: |
, 2018
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/5059552 |
Daftar Isi:
- Telomeres are specialized nucleoprotein structures that protect chromosome ends from DNA damage response (DDR) and DNA rearrangements. The telomeric shelterin protein TRF2 suppresses the DDR, and this function has been attributed to its abilities to trigger t-loop formation or prevent massive decompaction and loss of density of telomeric chromatin. Here, we applied stochastic optical reconstruction microscopy (STORM) to measure the sizes and shapes of functional human telomeres of different lengths and dysfunctional telomeres that elicit a DDR. Telomeres have an ovoid appearance with considerable plasticity in shape. Examination of many telomeres demonstrated that depletion of TRF2, TRF1, or both affected the sizes of only a small subset of telomeres. Costaining of telomeres with DDR markers further revealed that the majority of DDR signaling telomeres retained a normal size. Thus, DDR signaling at telomeres does not require decompaction. We propose that telomeres are monitored by the DDR machinery in the absence of telomere expansion and that the DDR is triggered by changes at the molecular level in structure and protein composition.
- FiguresScripts for generating figures and the final figuresfigure_environmentPython software environment for figure creationFigure 1 and S2 and S6Data for figures 1, S2, and S6Figure 2Data for figure 2Figure 3 and 4Data for figures 3 and 4Figure 3Data for figure 3Figure 5Data for figure 5Figure S1Data for figures S1Figure S3Data for figure S3Figure S4 and S6Data for figures S4 and S6Figure S4Data for figure S4Figure S5Data for figures S5