Data from: A heritable symbiont and host-associated factors shape fungal endophyte communities across spatial scales
| Main Authors: | Harrison, Joshua G., Parchman, Thomas L., Cook, Daniel, Gardner, Dale R., Forister, Matthew L. |
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| Format: | info dataset Journal |
| Terbitan: |
, 2019
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| Subjects: | |
| Online Access: |
https://zenodo.org/record/4989968 |
Daftar Isi:
- 1. Although microbial ecologists are intensely interested in the processes governing microbial community assembly, progress has been limited by a lack of studies that span multiple geographical scales and levels of biological organization. 2. We used high throughput sequencing to characterize foliar fungal endophyte communities and host plant genetic structure both within, and among, 24 populations of spotted locoweed (Astragalus lentiginosus) across the Great Basin Desert. 3. Across the Great Basin, both within, and among populations of the host plant, fungal endophyte richness was predicted by plant size and variation in the seed-borne, heritable fungus, Alternaria fulva, which produces the bioactive alkaloid swainsonine. 4. The degree of between-plant turnover in the endophyte community was inversely related to host plant inbreeding and average plant size, and positively related to the relative abundance of A. fulva. Plant size was inversely related to endophyte community richness, both among, and within populations. The genetic and physical distance between host populations was not predictive of differences in fungal community structure. 5. Synthesis: Through pairing intensive local- and regional sampling, we uncovered a primacy of deterministic forces imposed by a heritable symbiont on the community structure of locoweed endophytes.
- TaxonomicHypothesesTaxonomic hypotheses output from the UNITE and Warcup training databases. SINTAX was used to assign taxonomy. Confidence in assignments is shown. UNITE was accessed May 3, 2017 and Warcup was accessed Dec 4, 2017Sequences of OTUsFASTA file with sequencesFungalOTUs.faOTU tableOTU table, see main text and supplemental methods for details of how this table was made.asleOtuTable_sintax.csvLabelling keyWell to sample key. Samples were spread across eight 96 well plates (denoted p1–8 in field 1). Each well corresponded with a specific sample, the name of which is shown in field 2. The lane the sample was sequenced on is shown in field 3. Note that there were samples from a different project sequenced at the same time (see supplemental methods).Labellingkey.csvData for focal populationsSummary indices and abiotic information for each focal population. See "readme" file for information on what each field contains.populationLevelData.csvFunding provided by: National Science FoundationCrossref Funder Registry ID: http://dx.doi.org/10.13039/100000001Award Number: DEB-1050726 and DEB-1638793