KONSENTRASI PACLOBUTRAZOL DAN PEMISKINAN MEDIA PADA PELESTARIAN IN VITRO TANAMAN KRISAN (Chrysanthemum morifolium Ramat)
| Main Authors: | Demmassabu, Sofia, Kojoh, Diane A., Arsyad, Yulie P. |
|---|---|
| Format: | Article application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
EUGENIA
, 2014
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| Online Access: |
http://ejournal.unsrat.ac.id/index.php/eugenia/article/view/3537 |
Daftar Isi:
- ABSTRACT Research was conducted at biotechnology laboratory of Agriculture Faculty, Samratulangi University Manado since July 2009 until October 2009. The objective was to assess the effect of Paclobutrazol usage and MS media reducing in chrysan perpetuation through in vitro. The research was designed in randomized complete designed using factorial. First factor was basic media concentration of Murashige and Skoog with 2 concentration : M1 = 100% MS, M2 = 10% MS and second factor was paclobutrazol with 4 concentration s which were P0 = 0 ppm paclobutrazol (control), P1 = 1.0 ppm, P2 = 1.5 ppm, P3= 2.0 ppm, and P4 = 2.5 ppm paclobutrazol. The treatments were repeated ten times. Observed variables were crysan tall, the amount of root and buds that was measured at the end of research. The result showed that 100 % MS media, addition of paclobutrazol obviously produced shorter chrysan, and less amount of roots and buds compare to media without paclobutrazol. Media with 10 % MS combine with paclobutrazol 2.0 ppm produced shorter chrysan than media without paclobutrazol. The amount of roots and buds in media with paclobutrazol were not different with media without paclobutrazol. Key words : Paclobutrazol, crysan