Production, Characterisation, and Isolation of Lipase from Aspergillus niger by using Palm Oil as Inducer
| Main Authors: | Murni, Sri Wahyu, Kholisoh, Siti Diyar, D.L., Tanti, E.M., Petrissia |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | ind |
| Terbitan: |
Prodi Teknik Kimia, Fakultas Teknologi Industri, UPN "Veteran" Yogyakarta
, 2015
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| Subjects: | |
| Online Access: |
http://jurnal.upnyk.ac.id/index.php/eksergi/article/view/1034 http://jurnal.upnyk.ac.id/index.php/eksergi/article/view/1034/pdf_7 |
Daftar Isi:
- The objective of the research was to produce, isolate and characterise lipase from Aspergillus niger, and therefore inducted it by using palm oil. The lipase enzyme was produced through a batch fermentation process in a 1.4 liters-fermentor. Fermentation was carried out at room temperature, initial pH of 7, the stirring speed of 250 rpm, aeration rate of 1 vvm, and inducer concentration of 3%-m/v palm oil/ml. Enzymes was characterised at several temperature and pH variations. The lipase showed the optimum performance at pH of 7 and temperature of 30 °C with the activity of 1.5 U / ml. Isolation of lipase yielded a 4-times-increase in its activity by using 90% ammonium sulfate.
- Tujuan penelitian ini adalah memproduksi, mengisolasi, dan mengkarakterisasi lipase dengan induser minyak goreng sawit. Fermentasi submerged 1,4 L digunakan selama proses produksi pada suhu kamar, dengan pH awal 7, kecepatan pengadukan 250 rpm, kecepatan aerasi vvm, dan konsentrasi induser 3%-m/v minyak goreng sawit. Enzim dikarakterisasi pada variasi temperature dan pH. Lipase dihasilkan secara optimum pada pH 7 dan suhu 30 °C dengan aktivitas 1,5 u/ml. Isolasi lipase menghasilkan 4 kali peningkatan dengan menggunakan 90% amonium sulfat