The Role of CYP4502E1 Genetic Polymorphism on Benzene Metabolism
| Main Authors: | Dewi Sumaryani Soemarko; Department Community Medicine, Faculty of Medicine- Universitas Indonesia, Muchtaruddin Mansyur; Division of Occupational Medicine, Community Medicine department, Medical Faculty, School of Medicine, Universitas Indonesia, Septelia Inawati Wanandi, Novi Silvia Hardiany |
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| Format: | Article application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Faculty of Medicine Universitas Indonesia
, 2017
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| Subjects: | |
| Online Access: |
http://journal.ui.ac.id/index.php/eJKI/article/view/7430 |
Daftar Isi:
- Abstract: Benzene has genotoxic/carcinogenic characteristic which always make it interesting subject for research. The carcinogenic effect is also associated with metabolites produced in benzene metabolism such as phenol, catechol, quinones, muconic acid (tt-MA), and phenyl mercapturic acid (s-PMA). The role of CYP4502E1 enzyme in benzene metabolism is very important, which is determined by its genetic polymorphism. The presented cross-sectional study was aimed to obtain the distribution of frequency of s-PMA concentration in workers who had been low exposure of benzene based on CYP4502E1 genetic polymorphism. The study was conducted as a part of another study, i.e. the effect of low-level benzene exposure on the incidence of chromosomal breakage in 115 full-time workers at an oil company in Kalimantan between September 2007 and April 2010. Data were collected by methods of interviews, physical examinations, laboratory examinations, and direct observation on the work place. The variables studied were CYP4502E1, benzene exposure at the work place, age, type of work, history of work, length of work, body mass index (BMI), antioxidants intake, behavior and management, and s-PMA concentration in the urine. The distribution of CYP4502E1 genetic polymorphism in workers is 87.8% wild type homozygote, 11.3% heterozygote and 0.9% mutant homozygote. There was no difference in the proportion of s-PMA concentration based on CYP4502E1 genetic polymorphism (p=0.595; ORraw=0.98; 95% CI=0.95-1.01). There were also no differences of age, type of work, length of work, BMI, antioxidants consumptions, behavior and management of subjects with s-PMA. Further study should be performed on CYP4502E1 genetic polymorphism in various Indonesian races at different workplace with low-level benzene. Abstrak: Benzene memunyai karakter genotoksik/ karsinogeneik yang selalu menjadi subjek untuk penelitian. Efek karsinogenik juga berhubungan dengan proses metabolisme benzena yang menghasilkan metabolit seperti fenol, katekol, qinon,asam mukonik (tt-MA), dan asam fenil merkapturik (s-PMA). Peran enzim CYP4502E1 dalam metabolisme benzena sangat penting, yang ditentukan oleh polimorfisme genetiknya. Disain potong lintang pada studi ini bertujuan melihat distribusi konsentrasi s-PMA diantara pekerja yang terpajan benzena rendah berdasarkan polimorfisme genetik CYP4502E1. Studi ini merupakan bagian dari studi lain,yaitu Insiden patahan Kromosom akibat pajanan Benzena rendah diantara 115 pekerja tetap di perusahaan Migas di Kalimantan pada bulan September 2007 sampai April 2010. Data dikumpulkan dengan wawancara, pemeriksaan fisik, pemeriksaan laboratorium dan observasi langsung di lingkungan kerja. Variabel yang diukr adalah CYP4502E1, pajanan benzena di lingnkungan kerja, umur,jenis pekerjaan, riwayat pekerjaan, masa kerja, indeks massa tubuh, asupan antioksidan, kebiasaan dan manajemen, dan konsentrasi s-PMA dalam urin. Distribusi Polimorfisme genetik CTP4502E1 pada pekerja adalah 87.8% wild type homozygote, 11.3% heterozygote and 0.9% mutant homozygote. Tidak ada perbedaan proporsi antara konsentrasi s-PMA berdasarkan polimorfisme genetik CYP4502E1 (p=0.595; ORraw=0.98; 95% CI=0.95-1.01). Tidak ada juga perbedaan dari umur, jenis pekerjaan, masa kerja, indeks massa tubuh, konsumsi antioksidan, kebiasaan dan manajemen dari s-PMA pekerja. Penelitian lanjut perlu dilakukan untuk melihat polimorfisme genetik CYP4502E1 tiap suku bangsa di Indonesian di lingkungan kerja yang terpajan benzena rendah.