Phytochemical assay, potential of antimalarial and antioxidant activities of green tea extract and its fractions
| Main Authors: | Rahardja, Fanny, Tjokropranoto, Rita, Widowati, Wahyu, Lusiana, Siska, Suhendra, Adrian, Tjahjani, Susy, Budiman, Iwan, Maesaroh, Maesaroh, Fauziah, Nurul |
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| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Biomedical Engineering
, 2014
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| Online Access: |
https://be.ub.ac.id/index.php/jibe/article/view/4 https://be.ub.ac.id/index.php/jibe/article/view/4/4 |
Daftar Isi:
- Objective : The research objective was to evaluate the phytochemical compounds, the antioxidant and anti-malarial activity of green tea extract and its fraction. Methods: The antioxidant activity were measured by 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity used in vitro laboratories prospective experimental design and completely randomized design, using ethanol extract, hexane, ethyl acetate, butanol fraction, and water fraction ofgreen tea in 7 levels of concentration compared to epicatechin (EC). The data were analyzed using linear regression analysis, and were continued by determined Inhibitory Concentration). The anti-malarial activity of green tea fractions were compared to artemisinin. The data were analyzed using probit analysis, and were continued by determined IC. Results: The results showed that the highest antioxidant activity (IC50) was the fraction of ethyl acetate 2,184 μg/ml. The anti-malarial activity IC50 50 of water and ethyl acetate fraction, ethanol extract and butanol fraction were 0,000090 μg/mL,0,003 μg/mL, 0,006 μg/mL, 0,011 μg/ml respectively.Conclusion: Extract and fractions of green tea have potential anti-malarial and antioxidant properties. Key Words: antioxidant; anti-malarial; green tea; free radicals