Antioxidant and hypoglycemic activities of extract and fractions of Rambutan seeds (Nephelium lappaceum L.)
| Main Authors: | Soeng, Sylvia, Evacuasiany, Endang, Widowati, Wahyu, Fauziah, Nurul |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Biomedical Engineering
, 2014
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| Online Access: |
https://be.ub.ac.id/index.php/jibe/article/view/2 https://be.ub.ac.id/index.php/jibe/article/view/2/2 |
Daftar Isi:
- Objective : This research was done to measure the antioxidants and hypoglycemic activities of Nephelium lappaceum L. seed (NLS) extract and fractions. Methods: The ethanol extract of rambutan seeds were prepared by maseration method and the fractions (n-hexane, ethyl acetate, buthanol and water) by separation of extract based on the polarity. The antioxidant activity was determined by using superoxide dismutase value (SOD), 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging activity. The hypoglycemic activity was determined by using inhibition of α-glucosidase test. The DPPHscavenging and hypoglycemic activities were analized by median of Inhibitory Concentration (IC-50). Results: The highest SOD activity showed that ethyl acetate and aqueous fraction of NLS were 3.3771 μg/ml and 3.0374 μg/ml. Meanwhile DPPH assay showed that both NLS extract and fractions had low DPPH scavenging activity. Hypoglycemic activity showed that extract of NLS had highest activity as α-glucosidase inhibitor. Conclusion: Nephelium lappaceum L. seed extract and fractions have high SOD antioxidantvalue but low DPPH scavenging activity and can be used as potential hypoglycemic agent. Key Words: antidiabetic; antioxidants; α-glucosidase; hypoglycemic; scavenging