Diuretic Activity Test n-Hexane Extract of Ceplukan (Physalis angulata L.) Leaves on Wistar Male Rat (Rattus norvegicus)
| Main Authors: | FITRIANA, INDRAWATI, NUGROHO, AGUNG ENDRO, WIDODO, GUNAWAN PAMUDJI |
|---|---|
| Format: | Article info application/pdf Journal |
| Bahasa: | ind |
| Terbitan: |
Fakultas Farmasi Universitas Setia Budi
, 2020
|
| Online Access: |
http://ejurnal.setiabudi.ac.id/ojs/index.php/farmasi-indonesia/article/view/714 http://ejurnal.setiabudi.ac.id/ojs/index.php/farmasi-indonesia/article/view/714/560 |
Daftar Isi:
- Ceplukan (Physalis angulata L.) empirically have diuretic effect. This study aimed to determined the diuretic effect of n-hexane extract of ceplukan leaves on Wistar male rats. Ceplukan leaves powder were extracted by maceration with n-hexane solvent. Test animal was used 25 Wistar male rats were divided into 5 groups: group I were given CMCNa 0.5% (negative control), group II (positive control) were given furosemide dose 4.32 mg/200 g BW, group III, IV, V were given n-hexane extract suspension dose of 375, 187.5, 93.75 mg/200 g BW respectivelly. The urine volume were measured in hours-6, 12, 18 and 24. Diuretic effect is obtained by calculating the cumulative urine volume and AUC (Area Under the Curve). The data obtained were analyzed by one way ANOVA with a level of 95% significant, then test by LSD (Least Significant Difference).The results showed that n-hexane extract of ceplukan leaves had diuretic effect. The effective dose of n-hexane extract of ceplukan leaves for diuretic effect was dose 375 mg / 200 g BW.
- Ceplukan (Physalis angulata L.) secara empiris berkhasiat diuretik. Tujuan penelitian ini untuk menentukan efek diuretik ekstrak n-heksana daun ceplukan pada tikus jantan Wistar. Serbuk daun ceplukan diekstraksi secara maserasi dengan pelarut n-heksana. Hewan uji yang digunakan sebanyak 25 ekor tikus jantan galur Wistar yang dibagi menjadi 5 kelompok, yaitu kelompok I diberi CMC Na 0,5 % (kontrol negatif), kelompok II diberi furosemid dosis 4,32 mg/200 g BB (kontrol positif), kelompok III, IV, V diberi ekstrak n-heksana dosis berurut-turut 375; 187,5; 93,75 mg/200 g BB dengan volume pemberian 2,5 ml/200 g BB. Volume urin diukur pada jam ke-6, 12, 18, dan 24. Efek diuretik diperoleh dengan menghitung volume urin kumulatif dan AUC (Area Under the Curve). Data dianalisis dengan ANOVA satu jalan taraf kepercayaan 95%, kemudian dilakukan uji LSD (Least Significant Difference). Hasil penelitian menunjukkan bahwa ekstrak n-heksana daun ceplukan memiliki efek diuretik terhadap tikus jantan Wistar. Dosis efektif ekstrak n-heksana daun ceplukan untuk efek diuretik adalah 375 mg/200 g BB.