Effectiveness of Chinese Leaf Extract and Leaf Plants (Jatropha Multifida L.) As Antibacterial to Bacterial Growth Staphylococcus Aureus In Vitro
Main Authors: | Anggita, Dwi, Yusriani, Yusriani, Abdi, Dian Amelia, Desiani, Vivin |
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Format: | Article info application/pdf Journal |
Bahasa: | eng |
Terbitan: |
Fakultas Kesehatan Masyarakat Universitas Muslim Indonesia
, 2018
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Online Access: |
http://jurnal.fkmumi.ac.id/index.php/woh/article/view/woh1106 http://jurnal.fkmumi.ac.id/index.php/woh/article/view/woh1106/woh1106 |
Daftar Isi:
- Jatropha multifida L. contains alkaloids, tannins, flavonoids, saponins and phenolic acids that differ from each part of the plant and the content of these substances is what makes L. multifida Jatropha has a function as an antibacterial so that the leaf extract and the gap of chinese distance ( Jatropha multifida L.) allegedly capable of inhibiting the growth of Staphylococcus aureus bacteria. The aim of this research was to know the effectivity of leaf extract and gum of Jatropha multifida L. to the growth of Staphylococcus aureus bacteria in vitro.Penelitian used true experimental post test design. Using disc diffusion method with concentration 25; 50; 75; and 100% v / v leaf and gum china distance. Positive control was used Clindamycin 5μg drip antibiotic paper disc. The data were analyzed descriptively. The result of the research showed the difference of different inhibition zone between treatments. In chinese leaf extracts showed different mean diameters, at concentrations of 25%, 50%, 75% with inhibit zone formed respectively 0 mm, 9.32 mm, and 17.48 mm and the highest inhibition zone at concentration of 100% with an average diameter of 22.24 mm. In the gap of chinese distance showed a different mean diameter, at concentrations of 25%, 50%, 75% with the inhibit zone formed respectively 16.08 mm, 18.15 mm, and 18.63 mm and the highest inhibition zone at a concentration of 100% with an average diameter of 21.91 mm. Clindamycin positive controls show an average inhibit zone of 23.31 mm. It was concluded that there was an effect of leaf extract and resin of chinese distance plant (Jatropha multifida L.) on growth of Staphylococcus aureus bacteria, and the best concentration was 100% concentration and almost closer to the inhibitory zone of Clindamycin antibiotics as positive control.
- Tanaman jarak cina (Jatropha multifida L.) mengandung alkaloid, tanin, flavonoid, saponin dan asam fenolik yang berbeda dari setiap bagian tanamannya dan kandungan zat-zat tersebutlah yang membuat Jatropha multifida L. mempunyai fungsi sebagai antibakteri sehingga ekstrak daun dan getah jarak cina (Jatropha multifida L.) diduga mampu menghambat pertumbuhan bakteri Staphylococcus aureus. Penelitian bertujuan untuk mengetahui efektifitas ekstrak daun dan getah tanaman jarak cina (Jatropha multifida L.) terhadap pertumbuhan bakteri Staphylococcus aureus secara in vitro. Penelitian menggunakan desain penelitian true experimental post test. menggunakan metode disc diffusion dengan konsentrasi 25; 50; 75; dan 100% v/v daun dan getah jarak cina. Kontrol positif digunakan kertas cakram antibiotik Clindamycin 5μg. Data dianalisis secara deskriptif. Hasil penelitian menunjukkan adanya perbedaan zona hambat yang berbeda-beda antar perlakuan. Pada ekstrak daun jarak cina memperlihatkan rata-rata diameter yang berbeda, pada konsentrasi 25%, 50%,75% dengan zona hambat yang terbentuk masing-masing adalah 0 mm, 9,32 mm, dan 17,48 mm serta zona hambat tertinggi pada konsentrasi 100% dengan rata-rata diameter 22,24 mm. Pada getah jarak cina memperlihatkan rata-rata diameter yang berbeda, pada konsentrasi 25%, 50%,75% dengan zona hambat yang terbentuk masing-masing adalah 16,08 mm, 18,15 mm, dan 18,63 mm serta zona hambat tertinggi pada konsentrasi 100% dengan rata-rata diameter 21,91 mm. Kontrol positif Clindamycin memperlihatkan zona hambat rata-rata 23,31 mm. Disimpulkan bahwa terdapat pengaruh ekstrak daun dan getah tanaman jarak cina (Jatropha multifida L.) terhadap pertumbuhan bakteri Staphylococcus aureus, dan konsentrasi terbaik adalah konsentrasi 100% serta hampir mendekati zona hambat dari antibiotik Clindamycin sebagai kontrol positif.