UJI HAYATI FILTRAT BIAKAN JAMUR ENTOMOPATOGEN YANG DISINARI UV C TERHADAP LARVA Spodoptera litura (FABRICIUS) (LEPIDOPTERA: NOCTUIDAE) PADA CABAI (Capsicum annuum L.) = BIOASSAY OF THE FILTRATE OF ENTOMOPTHOGENIC FUNGAL CULTURE IRRADIATED WITH UV C AGAINTS LARVAE OF Spodoptera litura (FABRICIUS) (LEPIDOPTERA: NOCTUIDAE) ON CHILLI (Capsicum annuum L.)
Daftar Isi:
- Spodoptera litura is one of important pest of chili, belongs to Family Noctuidae and active during night (nocturnal). Entomopathogenic fungi such as Beauveria bassiana and Metarhizium anisopliae has been widely used to control S. litura. The use of secondary metabolite from entomopathogenic fungi is an alternative of insect control. B. bassiana produces toxin beauvericin, while M. anisopliae produces toxin destruxin. Therefore, tis research was aimed at knowing the effect of UV C 5, 10, 15 and 30 watt irradiations. The experiment was arranged in a Completely Randomized Block Design (CRBD) with 2 factors. The first factor was the fungi and the second was the isolate of UV C irradiation. Entomopathogenic fungi was incubated at room temperature for weeks to make culture filtrate and then irradiated with 5, 10, 15, 20 and 30 watt of UV C for 6 hours. Two isolates of entomopathogenic fungi was used, B. bassiana and M. anisopliae. The test insect was second instar of S. litura, 25 larvae per replicate. The obtained data was analyzed using ANOVA. The lethal time of individual larvae was used to determined LT50 using probit analyses. The results showed for factors of fungi species larval mortality entomopatogen teringgi found in a fungi in sinari UV C M. anisopliae with larval mortality (41.33%) with the death of the 12 days of with B. bassiana larval mortality ( 32.95%) with the death of 12 days, the shortest LT50 and the treatment in M. anisopliae with LT50 10 days and was significantly different from those under B. bassiana with LT50 of 18 days, interactions between different species of mushrooms and a very real meaning, certain yeast species influenced by a certain UV C shines. To factor very highest LT50 found on 5 watt LT50 shines 10 days but not significantly different from the shines with 10 watt 13 days, and 15 watt, 20 watt and was significantly different from those under with 21 days, 30 watts to 30 days. LT50 interactions between factors of fungal species and very w significantly different, LT50 shortest fungi M. anisopliae 5 watt shines with LT50 9 days, was significantly different from those with spesie fungal B. bassiana shines 20 watt LT50 27 days, and species B. bassiana shines with 30 watt LT50 42 days. Therefore fungi species M. anisopliae are UV irradiated 5 watts was the most effective in killing larvae of S. litura.