UJI ANTIBAKTERI RUMPUT LAUT Halimeda sp. DARI PERAIRAN TELUK HURUN LAMPUNG MENGGUNAKAN METODE EKSTRAKSI YANG BERBEDA
Daftar Isi:
- Halimeda sp. is a species of seaweed which known as antibacterial bioactive compound source. The sample of Halimeda sp. (H. renchii and H. xishaensis) were token from Gulf of Hurun, Lampung. The purposes of this research are to finding out the natural antibacterial of seaweed extract against bacteria of Staphyloccocus aureus and Escherichia coli, determining inhibiting zone of the bacterial growth using wet and dry methods, determining the minimum inhibitor concentration of Halimeda sp. extract. This research methodology was including the sample collecting of Halimeda sp. rarefaction wet and dry sample, drying sample, maseration, and extraction using ethyl acetate and methanol, evaporation, antibacterial test and Minimum Inhibiting Concentration (MIC) of Halimeda sp. This research result show that the highest antibacterial compound of Halimeda sp. is dry method with ethyl acetate as solvent the highest value of H. renchii’s antibacterial activiy is 7.42 mm from dry sample with ethyl acetate as solvent the using Escherichia coli and the lowest value is 6.26 mm from dry sample with methanol as solvent using Staphyloccocus aureus. The highest antibacterial activity of H. xishaensis is 9.42 mm from dry sample with methanol using Escherichia coli and lowest value is 6.10 mm from wet sample with ethyl acetate using both of the bacteria. The MIC value of H. renchii’s dry sample with ethyl acetate using Escherichia coli in 250 ppm concentration is 6.37 mm. The bacterial activity test of Halimeda sp. shows that the extract of H. renchii is stronger than H. xishaensis.