EFEK INHIBISI FRAKSI ETIL ASETAT DAUN BENALU KERSEN (Dendrophtoe pentandra (L.) Miq) TERHADAP ALPHA-GLUKOSIDASE
Daftar Isi:
- Background: Reasearch in antidiabetic drugs cotinues to be done because of the increasing number of diabetics each year. Several plants have been tested for their potential as antidiabetic, one of which is kersen leaves. However, reasearch on the parasitic leaves of kersen for its potential as an antidiabetic has not been conducted. This study aims to determinatine the inhibitory effect of the ethyl acetate fraction of the parasite leaves of kersen. Method: This researchs was an in vitro experimental analytic study conducted in a laboratory by testing the inhibitory effect if the enzyme alpha-glucosidase of the ethyl acetate fraction of the parasite leaves of kersen. Ethyl acetate fraction was obtained from liquid-liquid fraction of 96% ethanol extract using ethyl acetate solvent. Ethyl acetate fraction was made with several concentrations. The activity test of the ethyl acetate fraction of parasite leaves of kersen againts the inhibition of the alpha-glucosidase enzyme using p-nitrophenyl-alpha-D-glucoside substrate and alpha-glucosidase enzyme from Saccharomyces cerevisiae. The absorption test is measured with a spectrophotometry at a wavelength of 400 nm. Then the % inhibition and IC50 values were calculated using linier regression statistical test. Results: Secondary metabolites contained in the ethyl acetate fraction of the parasite leaves of kersen are alkaloids, flavonoids, triterpenoids, tanins, and saponins. The results of the testing of the inhibitory activity of the enzme alpha glucosidase ethyl acetate fraction of parasite leaves of kersen had an IC50 values of 42,61 ppm and acarbose used as a comparison had an IC50 values of 20,23 ppm. Conclusion: The ethyl acetate fraction of parasite leaves of kersen has an inhibitory effect of the enzyme alpha-glucosidase which is categorized as active as antidiabetic.