Artikel: Production and Characterization of Protease from TP2 isolate from Plant Swamp Silage

Main Authors: Baehaki, Ace, Lestari, Shanti Dwita, Tirtayasa, Agung, Hidayat, Arif, Gofar, Nuni
Format: Article PeerReviewed Book
Bahasa: eng
Terbitan: Research Journal of Biotechnology , 2018
Subjects:
Online Access: http://repository.unsri.ac.id/21698/1/ARTIKEL%2017%20UNTUK%20PEER%20REVIEW%2017.%20Production%20and%20characterization%20of%20protease%20from%20TP2%20isolate.pdf
http://repository.unsri.ac.id/21698/
Daftar Isi:
  • The purpose of this research was production and characterization of protease from TP2 isolate of Plant Swam Silage. The optimum pH and temperature of protease from TP2 isolate were 11.0 and 45oC respectively. Na+ and Mg2+ increased TP2 protease whereas K+, Fe2+ and Zn2+ inhibited protease from TP2 isolate inhibiting the enzyme. Study on the effect of metals ion indicated that protease from TP2 isolate was metaloenzyme. Moleculer weight of protease by using SDSPAGE from TP2 isolate was 34,75 kD to 185,51kDa.