FORMULASI DAN KARAKTERISASI SEDIAAN INTRANASAL LIPOSOM ASAM VALPROAT UNTUK TERAPI EPILEPSI DENGAN VARIASI KONSENTRASI KOLESTEROL
Daftar Isi:
- The purpose of this study was to make a intranasal liposomes formulation and characterization of valproic acid for epilepsy theraphy using variations of cholesterol concentration. Variations concentration of cholesterol were carried out to determine the characteristics of liposomes valproic acid then. Liposomes preparations using hydration thin layer method and characterized by oraganoleptis, pH, viscosity, percent EE, and stability. The optimum formula was obtained at concentration cholesterol 42,11 mg and concentration soya lecithin 379 mg. Percent efficiency entrapment was conducted to find out how much drug entrapment in the vesicle and the result shows that vesicle contains 93,8172±0,0672% with %CV value of 0,0716% which means that result obtained uniform. Liposomes are characterized using Particle Size Analyzer to know particle size, Poly Dispersity Index and potential zeta value. The optimum formula produces particle size of 682,9 nm with PDI values of 0,380 and potential zeta of -71,11 mV. Diffusion test ex vivo was performed using Franz diffusion cell conducted to determine the power penetration liposomes and pure valproic acid. From the ex vivo drug diffusion analysis, it was found that 56,3066% of liposomes and pure valproic acid only penetrated 38,8006%. Evaluation results indicate that valproic acid have good characteristics as intranasal preparations.