UJI AKTIVITAS ANTIDIABETES EKSTRAK ETANOL DAUN KOPI ROBUSTA (Coffea canephora) TERHADAP TIKUS PUTIH JANTAN GALUR WISTAR YANG DIINDUKSI ALOKSAN
Daftar Isi:
- Antidiabetic activity test of ethanolic extract coffee robusta‟s leaves (Coffea canephora) to rats induced by alloxan has been done. Male wistar albino rats are used as animal models which divided into 6 groups, normal group (aquadest), negative control group (Na CMC 0,5%), positive control group (glibenclamide 0,43 mg/200 gBB), and 3 test group (ethanolic extract of coffee robusta‟s leave 53,5, 107, dan 214 mg/kgBB). Rats blood glucose level after induced intraperitoneally by alloxan 130 mg/kgBB can be stated as diabetic when >200 mg/dL. Preprandial blood glucose levels are measured using DTN-410-K photometer, on day 0, 5, 10, and 15. The result of AUC0-15 and percentage of decreasing blood sugar levels for positive control group are 2747,52 and 37,95%, and 3 treatment groups (53,5 mg/kgBB, 107 mg/kgBB, and 214 mg/kgBB) 3598,32 and 17,59%; 3275,80 and 25,00%; 2770,05 and 36,53%. This point indicated that the ethanolic extract of coffee robusta‟s leaves has an antidiabetic activity and there is no significant difference compared with glibenclamide (p<0,05). Percentage of blood glucose decrease level the third treatment group there is no significant difference compare with positive control group. According to the relation between percentage of blood glucose decrease level with dose, value of ED50 of ethanolic extract of coffee robusta‟s leaves is 327,68 mg/kgBB.