PREPARASI DAN KARAKTERISASI SUBMIKRO PARTIKEL POLY(LACTIC-CO-GLYCOLIC ACID) PEMBAWA KLINDAMISIN DENGAN VARIASI KECEPATAN ULTRA TURRAX
Daftar Isi:
- Preparation of submicro particles aims to increase clindamycin penetration into the skin intended to inhibit the growth of acne caused of bacteria P. acnes was effectively. Preparation of clindamycin in the form of submicro particles consisted of PLGA as polimer which were stabilized by PVA using emulsion solvent evaporation method. Submicro particles component was consisted of clindamycin, PLGA, PVA, and ethyl acetate using ultra turrax with speed variation at 11.000, 13.000, and 15.000 rpm. All three formulas consecutively generate entrapment efficiency percentage (%EE) of 67,70 ±2,128%; 66,833±4,901%; and 71,40±1,539%. The third formula using ultra turrax 15.000 rpm produced the highest EE percentage so that the third formula was the optimum formula. The result of submicro particles characterization such as diameter and particles distribution (Poly Dispersity Index/PDI) and potential zeta using Particle Size Analyzer (PSA) were 413,3 nm; 0,244; and -17,2 mV. Morphology particle of optimum formula was observed using Transmission Electron Microscope (TEM) showed spheric particles and homogen which PDI of 0,125. Particles permeability was observed through in vitro diffusion testing using Franz diffusion cell. The result of diffusion test during 24 hours showed the highest diffusion of PLGA-clindamycin submicro particles was 18,252±0,302%, compared with pure clindamycin solution and comercial clindamycin dosage form with diffusiom percentage each of 14,379±0,145% and 15,105±0,013%. The compartment analysis of submicro particles optimum formula showed that this formula followed two compartment (without lag time). Shelf life and half life testing from optimum formula using variation in temperature of 40; 50; and 60oC produced a shelf life for 29 days and half life 260 days with the test duration during 2 days than pure clindamycin solution which decreased level of 90% within 5 hours at 60oC. Expiration time of submicro particles was longer and could be improved the permeability.