Figure 1D - tRNA acylation levels of exponentially growing MG1655 and MG1655relA::HTF measured by Northern blotting analysis
| Main Author: | Gerdes, Kenn |
|---|---|
| Format: | Dataset |
| Terbitan: |
Mendeley
, 2018
|
| Subjects: | |
| Online Access: |
https:/data.mendeley.com/datasets/vv3pvnb75h |
| ctrlnum |
0.17632-vv3pvnb75h.1 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc><creator>Gerdes, Kenn</creator><title>Figure 1D - tRNA acylation levels of exponentially growing MG1655 and MG1655relA::HTF measured by Northern blotting analysis</title><publisher>Mendeley</publisher><description>tRNA acylation levels of exponentially growing MG1655 and MG1655relA::HTF measured by northern blotting analysis. Total tRNA was extracted from cells growing in MOPS minimal medium with 0.2% glucose as the carbon source before and after isoleucine starvation. A fraction of the purified tRNA was deacylated (by 100 mM Tris-HCL [pH 9]) and charged and uncharged samples were separated by urea PAGE and transferred to a membrane. tRNAileTUV, tRNAasnUTVW and tRNAtrpT were detected by hybridization with radiolabeled tRNA-specific DNA oligonucleotide probes.</description><subject>Southern Blot Analysis</subject><type>Other:Dataset</type><identifier>10.17632/vv3pvnb75h.1</identifier><rights>Creative Commons Attribution 4.0 International</rights><rights>http://creativecommons.org/licenses/by/4.0</rights><relation>https:/data.mendeley.com/datasets/vv3pvnb75h</relation><date>2018-04-04T13:45:53Z</date><date>2018-04-04T22:00:00Z</date><recordID>0.17632-vv3pvnb75h.1</recordID></dc>
|
| format |
Other:Dataset Other |
| author |
Gerdes, Kenn |
| title |
Figure 1D - tRNA acylation levels of exponentially growing MG1655 and MG1655relA::HTF measured by Northern blotting analysis |
| publisher |
Mendeley |
| publishDate |
2018 |
| topic |
Southern Blot Analysis |
| url |
https:/data.mendeley.com/datasets/vv3pvnb75h |
| contents |
tRNA acylation levels of exponentially growing MG1655 and MG1655relA::HTF measured by northern blotting analysis. Total tRNA was extracted from cells growing in MOPS minimal medium with 0.2% glucose as the carbon source before and after isoleucine starvation. A fraction of the purified tRNA was deacylated (by 100 mM Tris-HCL [pH 9]) and charged and uncharged samples were separated by urea PAGE and transferred to a membrane. tRNAileTUV, tRNAasnUTVW and tRNAtrpT were detected by hybridization with radiolabeled tRNA-specific DNA oligonucleotide probes. |
| id |
IOS7969.0.17632-vv3pvnb75h.1 |
| institution |
Universitas Islam Indragiri |
| affiliation |
onesearch.perpusnas.go.id |
| institution_id |
804 |
| institution_type |
library:university library |
| library |
Teknologi Pangan UNISI |
| library_id |
2816 |
| collection |
Artikel mulono |
| repository_id |
7969 |
| city |
INDRAGIRI HILIR |
| province |
RIAU |
| shared_to_ipusnas_str |
1 |
| repoId |
IOS7969 |
| first_indexed |
2020-04-08T08:14:17Z |
| last_indexed |
2020-04-08T08:14:17Z |
| recordtype |
dc |
| _version_ |
1686587405177454592 |
| score |
17.538404 |