Gene expression in primary neurons upon knockdown of Diras2
| Main Author: | Freudenberg, Florian |
|---|---|
| Other Authors: | Grünewald, Lena , Chiocchetti, Andreas G, Weber, Heike, Scholz, Claus-Jürgen, Schartner, Christoph, Reif, Andreas |
| Format: | Dataset |
| Terbitan: |
Mendeley
, 2018
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| Subjects: | |
| Online Access: |
https:/data.mendeley.com/datasets/rtsx7zpt4w |
Daftar Isi:
- The expression of 88 target genes and three reference genes (Pgk1, Rpl13a and Sdha) was analyzed in singlets using a RealTime ready Custom Panel 384 – 96+ with Light Cycler 480 Probes Master on a LightCycler480 (Roche) using the following conditions: i) 95 °C for 10 min, ii) 45 cycles of 95 °C for 10 s, 60 °C for 30 s, 72 °C for 1 s co-terminated by a plate read. AAV2 = cells treated with AAV-U6-Diras2_2-hSyn::mCherry.3xFLAG-WPRE AAV4 = cells treated with AAV-U6-Diras2_1-hSyn::mCherry.3xFLAG-WPRE Scr = cells treated with AAV-U6-Scrambled-hSyn::mCherry.3xFLAG-WPRE Unb = untreated cells