Production and chracterization a novel exopolysaccharide produced by Lactobacillus plantarum W1 isolated from soybean whey
| Main Author: | Dothibich, Thuy |
|---|---|
| Format: | Dataset |
| Terbitan: |
Mendeley
, 2018
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| Subjects: | |
| Online Access: |
https:/data.mendeley.com/datasets/my57rxvbww |
Daftar Isi:
- Lactic acid bacteria (LAB) are a group of beneficial bacteria widely used by the food industry around the world. Beside the lactic acid production, their biosynthesis of enzymes, bacteriocin and exopolysaccharides are used to produce probiotics. Exopolysaccharides of lactic acid bacteria have many characteristics that can be used as food additives, especially in improving the appearance and stabilizing the volatility of dairy products. In this research, Lactobacillus plantarum W1 (EPS-W1) isolated from soybean whey was identified by Phenylalanyl-tRNA sequencing method. Its strain number is R-49778 in BCCM/LMG Bacteria collection, Ghent University, Belgium.This strain had the highest ability of exopolysaccharide synthesis among the examined strains. Gel permeation chromatography revealed that the exopolysaccharide of this strain has average molecular weight of 1.11x105 Da. The result of gas–liquid chromatography–mass spectrometry analyses showed that it’s monosaccharide composition included of glucose and mannose in 1.49:1 ratio. By 1D, 2D nuclear magnetic resonance spectroscopy the repeating unit of this polysaccharide to be shown -D-(1 → 6)-linked glucosyl,-D-(1 → 3)-linked mannosyl, -D-(1 → 3)-linked glucosyl and branch of -D-(1→6)-linked mannosyl, -D-(1 → 2)-linked glucosyl. Comparison with previously study of EPS structure indentification showed that this is a new exopolysaccharide.