Gene resistance to transcriptional reprogramming following nuclear transfer is directly mediated by multiple chromatin repressive pathways. Supplementary Files (Replicate 1/2/4)

Main Author: Beaujean, Nathalie
Format: Dataset
Terbitan: Mendeley , 2017
Subjects:
Online Access: https:/data.mendeley.com/datasets/jzjspk58nv
ctrlnum 0.17632-jzjspk58nv.2
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format Other:Dataset
Other
author Beaujean, Nathalie
title Gene resistance to transcriptional reprogramming following nuclear transfer is directly mediated by multiple chromatin repressive pathways. Supplementary Files (Replicate 1/2/4)
publisher Mendeley
publishDate 2017
topic Microscopy
url https:/data.mendeley.com/datasets/jzjspk58nv
contents Apotome Images (raw data) of 2-cell fertilized mouse embryos with USP21 mRNA injection (inj) or not (T). Embryos are injected at fertilization with mRNA encoding USP21 and subsequently fixed for immunolabelling at the 2-cell stage with anti-H2AK119ub primary antibody (Cell Signaling #8240S + anti-rabbit Cy3-conjugated secondary antibody in red) and DAPI DNA conterstaining (in blue). A total number of 5 replicates were performed on 0140207, 20140211, 20140327, 20140505 and 20140506 (YYYY-MM-DD). In the three first experiments (Replicates 1/2/3), an internal microinjection reporter (HA-tag in green) was also included. In the last two replicates (20140505 and 20140506), co-detection of RING1B protein (Active Motif #39663 + anti-mouse FITC-conjugated secondary antibody in green) was included. Images file format: .zvi
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institution Universitas Islam Indragiri
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first_indexed 2020-04-08T08:22:13Z
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