Nitrogen isotopes, carbon isotopes, and pyrolysis products in wood decay fungi, woody tissues, and cellulose from a long-term log decomposition experiment at H.J. Andrews Experimental Forest, Oregon, USA
| Main Author: | Hobbie, Erik A. |
|---|---|
| Other Authors: | Grandy, A. Stuart, Harmon, Mark |
| Format: | Dataset |
| Terbitan: |
Mendeley
, 2019
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| Subjects: | |
| Online Access: |
https:/data.mendeley.com/datasets/hwtz5j2k7c |
Daftar Isi:
- We combined elemental, isotopic, and compositional patterns in wood, cellulose, and sporocarps to investigate functional and isotopic differences in six taxa of decay fungi during log decomposition. Fungal protein was 4-5‰ higher in δ15N and 3-4‰ higher in δ13C than non-protein. Fungal δ15N correlated with the proportion of protein in N-containing pyrolysis products. 15N partitioning between protein and non-protein pools in mycelia prior to sporocarp formation controlled sporocarp δ15N relative to N sources. Radiocarbon measurements separated fungi into heartwood colonizers (Fomitopsis and Hericium, ~30+-year-old carbon) and sapwood colonizers (Mycena, Hypholoma, and Trametes, 1-12-year-old carbon). Sporocarps were 0-2.5‰ higher in δ13C than wood cellulose; this was attributed to compositional differences, assimilation of some 13C-enriched hemicellulose or sucrose, and 13C discrimination during metabolism. Research highlights included: (1) Strategies of carbon and nitrogen acquisition differ among wood decay fungi, (2) Fungal taxa varied widely in chemical composition as assessed by pyrolysis GC-MS, (3) Fungal carbon was from one (Mycena) to 30+ years (Fomitopsis, Hericium) old, (4) Hericium preferentially assimilated 13C-enriched hemicellulose, (5) The removal of 13C-depleted C6 atoms in pentoses causes high 13C in hemicellulose, (6) taxa varied in N partitioning among sporocarps, mycelia, protein, and non-protein. From these measurements, we improved the quantitative and conceptual understanding of how sources, composition and metabolic processing determined isotopic composition of fungi.