Western Blot analysis of WT and LSD1-GT mES cells
| Main Author: | Agarwal, Saurabh |
|---|---|
| Format: | Dataset |
| Terbitan: |
Mendeley
, 2017
|
| Subjects: | |
| Online Access: |
https:/data.mendeley.com/datasets/d9876prwnx |
| ctrlnum |
0.17632-d9876prwnx.1 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc><creator>Agarwal, Saurabh</creator><title>Western Blot analysis of WT and LSD1-GT mES cells.</title><publisher>Mendeley</publisher><description>Western Blots were conducted whole cell lysates on WT mES and LSD1-GT mES cells with antibodies against various histone modifications and LSD1 (KDM1A). Cells were lysed with 2X Laemmli Buffer and were loaded at various concentrations on 12% acrylamide gels and probed with the following antibodies.
anti-H3K4me1 (ab8895, Abcam)
anti-H3K4me2 (ab7766, Abcam)
anti-H3K4me3 (ab8580, Abcam)
anti-H3K27ac (39135, Active Motif), and
anti-LSD1 (ab17721, Abcam)
For all images,
Lane 1: WT mES 3ul
Lane 2: WT mES 6ul
Lane 3: WT mES 12ul
Lane 4: LSD1-GT mES 3ul
Lane 5: LSD1-GT mES 6ul
Lane 6: LSD1-GT mES 12ul
Protein ladder was loaded on the lane left to Lane 1</description><subject>Western Blot</subject><type>Other:Dataset</type><identifier>10.17632/d9876prwnx.1</identifier><rights>Creative Commons Attribution 4.0 International</rights><rights>http://creativecommons.org/licenses/by/4.0</rights><relation>https:/data.mendeley.com/datasets/d9876prwnx</relation><date>2017-02-18T12:10:11Z</date><date>2018-02-18T12:09:41Z</date><recordID>0.17632-d9876prwnx.1</recordID></dc>
|
| format |
Other:Dataset Other |
| author |
Agarwal, Saurabh |
| title |
Western Blot analysis of WT and LSD1-GT mES cells |
| publisher |
Mendeley |
| publishDate |
2017 |
| topic |
Western Blot |
| url |
https:/data.mendeley.com/datasets/d9876prwnx |
| contents |
Western Blots were conducted whole cell lysates on WT mES and LSD1-GT mES cells with antibodies against various histone modifications and LSD1 (KDM1A). Cells were lysed with 2X Laemmli Buffer and were loaded at various concentrations on 12% acrylamide gels and probed with the following antibodies.
anti-H3K4me1 (ab8895, Abcam)
anti-H3K4me2 (ab7766, Abcam)
anti-H3K4me3 (ab8580, Abcam)
anti-H3K27ac (39135, Active Motif), and
anti-LSD1 (ab17721, Abcam)
For all images,
Lane 1: WT mES 3ul
Lane 2: WT mES 6ul
Lane 3: WT mES 12ul
Lane 4: LSD1-GT mES 3ul
Lane 5: LSD1-GT mES 6ul
Lane 6: LSD1-GT mES 12ul
Protein ladder was loaded on the lane left to Lane 1 |
| id |
IOS7969.0.17632-d9876prwnx.1 |
| institution |
Universitas Islam Indragiri |
| affiliation |
onesearch.perpusnas.go.id |
| institution_id |
804 |
| institution_type |
library:university library |
| library |
Teknologi Pangan UNISI |
| library_id |
2816 |
| collection |
Artikel mulono |
| repository_id |
7969 |
| city |
INDRAGIRI HILIR |
| province |
RIAU |
| shared_to_ipusnas_str |
1 |
| repoId |
IOS7969 |
| first_indexed |
2020-04-08T08:33:08Z |
| last_indexed |
2020-04-08T08:33:08Z |
| recordtype |
dc |
| _version_ |
1686587771369553920 |
| score |
17.538404 |