High-speed single molecule imaging of membrane proteins in rat basophilic leukemia cells
| Main Author: | Lidke, Keith |
|---|---|
| Format: | Dataset |
| Terbitan: |
Mendeley
, 2020
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| Subjects: | |
| Online Access: |
https:/data.mendeley.com/datasets/d5z3gt7xs9 |
Daftar Isi:
- A high-speed fluorescence microscope operating at a 490 Hz frame rate was used to image two different membrane proteins- the high-affinity IgE receptor FcεRI, a transmembrane protein, and an outer-leaflet GPI-anchored protein. The IgE receptor was imaged via IgE labeled with Janelia Fluor 646 and the GPI-anchored protein was imaged using a GPI-GFP fusion protein and an ATTO647N labeled anti-GFP nanobody. Data was collected for both proteins in untreated cells and cells that had actin stabilized by phalloidin.