Data for: Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider Parasteatoda tepidariorum
| Main Author: | Oda, Hiroki |
|---|---|
| Other Authors: | Akiyama-Oda, Yasuko |
| Format: | Dataset |
| Terbitan: |
Mendeley
, 2019
|
| Subjects: | |
| Online Access: |
https:/data.mendeley.com/datasets/c7cfhyd2p3 |
| ctrlnum |
0.17632-c7cfhyd2p3.3 |
|---|---|
| fullrecord |
<?xml version="1.0"?>
<dc><creator>Oda, Hiroki</creator><title>Data for: Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider Parasteatoda tepidariorum</title><publisher>Mendeley</publisher><description>Simultaneous, parental RNA interference (pRNAi) mediated knockdown of Hedgehog and Decapentaplegic (Dpp) signaling components, Pt-patched (Pt-ptc) and Pt-dpp, respectively, exhibited serious defects in the formation of the major embryonic axes in the model spider Parasteatoda tepidariorum. This paper describes a dataset of a custom oligonucleotide two-colour microarray analysis that was carried out to compare the transcript expression levels between untreated (normal) and Pt-ptc + Pt-dpp double pRNAi embryos at late stage 5. Array spots that showed the intensity ratio of [Pt-ptc + Pt-dpp double pRNAi]/[normal] <0.6 were categorized as positive. The expressions of most, not all, of the transcripts related to the positive array spots were examined in embryos by whole-mount in situ hybridization. Some of the stained embryos showed distinct patterns of gene expression. The data presented may be useful for characterizing the mechanisms of embyronic patterning in spider embryos.</description><subject>Arthropoda</subject><subject>Embryogenesis</subject><subject>Gene Expression</subject><subject>In Situ Hybridization</subject><subject>Pattern Formation</subject><subject>Oligonucleotide Microarrays</subject><subject>Model Organism</subject><subject>Animal Embryo</subject><contributor>Akiyama-Oda, Yasuko</contributor><type>Other:Dataset</type><identifier>10.17632/c7cfhyd2p3.3</identifier><rights>Creative Commons Attribution 4.0 International</rights><rights>http://creativecommons.org/licenses/by/4.0</rights><relation>https:/data.mendeley.com/datasets/c7cfhyd2p3</relation><date>2019-12-09T05:19:39Z</date><recordID>0.17632-c7cfhyd2p3.3</recordID></dc>
|
| format |
Other:Dataset Other |
| author |
Oda, Hiroki |
| author2 |
Akiyama-Oda, Yasuko |
| title |
Data for: Dataset on gene expressions affected by simultaneous knockdown of Hedgehog and Dpp signaling components in embryos of the spider Parasteatoda tepidariorum |
| publisher |
Mendeley |
| publishDate |
2019 |
| topic |
Arthropoda Embryogenesis Gene Expression In Situ Hybridization Pattern Formation Oligonucleotide Microarrays Model Organism Animal Embryo |
| url |
https:/data.mendeley.com/datasets/c7cfhyd2p3 |
| contents |
Simultaneous, parental RNA interference (pRNAi) mediated knockdown of Hedgehog and Decapentaplegic (Dpp) signaling components, Pt-patched (Pt-ptc) and Pt-dpp, respectively, exhibited serious defects in the formation of the major embryonic axes in the model spider Parasteatoda tepidariorum. This paper describes a dataset of a custom oligonucleotide two-colour microarray analysis that was carried out to compare the transcript expression levels between untreated (normal) and Pt-ptc + Pt-dpp double pRNAi embryos at late stage 5. Array spots that showed the intensity ratio of [Pt-ptc + Pt-dpp double pRNAi]/[normal] <0.6 were categorized as positive. The expressions of most, not all, of the transcripts related to the positive array spots were examined in embryos by whole-mount in situ hybridization. Some of the stained embryos showed distinct patterns of gene expression. The data presented may be useful for characterizing the mechanisms of embyronic patterning in spider embryos. |
| id |
IOS7969.0.17632-c7cfhyd2p3.3 |
| institution |
Universitas Islam Indragiri |
| affiliation |
onesearch.perpusnas.go.id |
| institution_id |
804 |
| institution_type |
library:university library |
| library |
Teknologi Pangan UNISI |
| library_id |
2816 |
| collection |
Artikel mulono |
| repository_id |
7969 |
| city |
INDRAGIRI HILIR |
| province |
RIAU |
| shared_to_ipusnas_str |
1 |
| repoId |
IOS7969 |
| first_indexed |
2020-04-08T08:16:10Z |
| last_indexed |
2020-04-08T08:16:10Z |
| recordtype |
dc |
| _version_ |
1686587413789409280 |
| score |
17.538404 |