ImageJ macro for semi-automated counting of nuclei

Main Author: Holian, Andrij
Other Authors: Trout, Kevin
Format: Dataset
Terbitan: Mendeley , 2019
Subjects:
Online Access: https:/data.mendeley.com/datasets/9fsh4w2s5x
Daftar Isi:
  • This semi-automated nuclei counting method gives the user an opportunity to qualitatively review output results for accuracy. The method was designed to work with fluorescently stained nuclei. If desired, the macro could be modified or applied to other staining methods. Although there are a variety of possible methods to count nuclei (e.g. machine learning), the goal of the method presented here was to run quickly and accurately on a basic consumer-level computer with freely available software (ImageJ).