Figure S1F - Western blot analysis of RelA protein levels before and after starvation
| Main Author: | Gerdes, Kenn |
|---|---|
| Format: | Dataset |
| Terbitan: |
Mendeley
, 2018
|
| Subjects: | |
| Online Access: |
https:/data.mendeley.com/datasets/8pf79gx3vk |
| ctrlnum |
0.17632-8pf79gx3vk.1 |
|---|---|
| fullrecord |
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<dc><creator>Gerdes, Kenn</creator><title>Figure S1F - Western blot analysis of RelA protein levels before and after starvation</title><publisher>Mendeley</publisher><description>Western blot analysis of RelA protein levels before and after starvation. MG1655, MG1655relA::HTF, MG1655relAΔRRM::HTF, MG1655relAΔZFDΔRRM::HTF, MG1655relAH432E::HTF where grown exponentially in MOPS minimal medium with 0.2% glucose at 30°C. Before starvation samples (1 mL) were collected at OD600 = 0.2 and the cultures starved for isoleucine by addition of L-Valine to a final concentration of 500 μg/mL. After 30 min of starvation, a second sample was collected. The samples were denatured and separated by SDS-PAGE, transferred to a PVDF membrane and the hexa Histidine tagged protein detected using pentahis antibodies.</description><subject>Western Blot</subject><type>Other:Dataset</type><identifier>10.17632/8pf79gx3vk.1</identifier><rights>Creative Commons Attribution 4.0 International</rights><rights>http://creativecommons.org/licenses/by/4.0</rights><relation>https:/data.mendeley.com/datasets/8pf79gx3vk</relation><date>2018-04-04T13:47:18Z</date><date>2018-04-04T22:00:00Z</date><recordID>0.17632-8pf79gx3vk.1</recordID></dc>
|
| format |
Other:Dataset Other |
| author |
Gerdes, Kenn |
| title |
Figure S1F - Western blot analysis of RelA protein levels before and after starvation |
| publisher |
Mendeley |
| publishDate |
2018 |
| topic |
Western Blot |
| url |
https:/data.mendeley.com/datasets/8pf79gx3vk |
| contents |
Western blot analysis of RelA protein levels before and after starvation. MG1655, MG1655relA::HTF, MG1655relAΔRRM::HTF, MG1655relAΔZFDΔRRM::HTF, MG1655relAH432E::HTF where grown exponentially in MOPS minimal medium with 0.2% glucose at 30°C. Before starvation samples (1 mL) were collected at OD600 = 0.2 and the cultures starved for isoleucine by addition of L-Valine to a final concentration of 500 μg/mL. After 30 min of starvation, a second sample was collected. The samples were denatured and separated by SDS-PAGE, transferred to a PVDF membrane and the hexa Histidine tagged protein detected using pentahis antibodies. |
| id |
IOS7969.0.17632-8pf79gx3vk.1 |
| institution |
Universitas Islam Indragiri |
| affiliation |
onesearch.perpusnas.go.id |
| institution_id |
804 |
| institution_type |
library:university library |
| library |
Teknologi Pangan UNISI |
| library_id |
2816 |
| collection |
Artikel mulono |
| repository_id |
7969 |
| city |
INDRAGIRI HILIR |
| province |
RIAU |
| shared_to_ipusnas_str |
1 |
| repoId |
IOS7969 |
| first_indexed |
2020-04-08T08:12:37Z |
| last_indexed |
2020-04-08T08:12:37Z |
| recordtype |
dc |
| _version_ |
1686587395163553792 |
| score |
17.538404 |