Recombinase A gene of rhizobium bacteria isolated from root nodules of bambara groundnuts [Vigna subterranea L. Verdc.] in Lake Victoria basin
| Main Author: | Onyango, Benson |
|---|---|
| Format: | Dataset |
| Terbitan: |
Mendeley
, 2018
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| Subjects: | |
| Online Access: |
https:/data.mendeley.com/datasets/78nm9gjv6m |
Daftar Isi:
- Recombinase A gene for rhizobium bacteria isolated form root nodules of bambara groundnuts [Vigna subterranea L. Verdc.] in Lake Victoria basin. RecA genes had uniform sizes of about 530 base pairs . The NCBI accession numbers of recA genes of isolates from this study are given (KJ736760 toKJ736764). Partial sequences of recA genes were used to construct a phylogenetic tree. Two groups A and B were obtained with Group A constituting two clades AI and AII. Clade AI had isolates BAMsp2, BAMKar1, BAMsp2 and BAMkis2 all clustered at 100% bootstrap support with Rhizobium sp. strain VIAD13G and PEPSM18. Isolate BAMKbay2 appeared to form a separate sub-clade indicating its slight genetic difference with the former isolates. Isolates BAMKbay1 and BAMKbay9 strongly clustered with Agrobacterium tumefaciens strain 11-17T B5 at 100% bootstrap support. The second sub-clade AII consisted of BAMKis1 and BAMKar2 which clustered with Rhizobium tropici (NCBI HF563598) and Rhizobium sp. strains BICC 651R and NCPPB 4062 at 99% support. This clustering trend indicated the likelihood of the two latter strains being within the species Rhizobium tropici. Group B had isolates BAMKis8 and BAMKis12 which clustered Bradyrhizobium elkanii strain C9 at 98% similarity index. An equal gene length of recA gene sequence of Pseudomonas sp. strain L1-44-08 was used as an out-group to root the phylogenetic tree.