Dataset for "Multiplexed Digital Detection of B-ALL Leukemia Fusion Transcripts using the NanoString nCounter System" Publication
| Main Author: | Zhong, Yunan |
|---|---|
| Format: | Dataset |
| Terbitan: |
Mendeley
, 2019
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| Subjects: | |
| Online Access: |
https:/data.mendeley.com/datasets/2bhgbvgn9p |
Daftar Isi:
- This dataset contains the validation data and analysis result for designing and developing a barcode-based nanoString nCounter technology for multiplexed detection of B-ALL leukemia fusion transcripts. Chromosomal rearrangements resulting in fusion transcripts have been reported in precursor B-cell acute lymphoblastic leukemia (B-ALL). Identification of fusion events is critical in the diagnosis and treatment of B-ALL. In this study, we used nanoString technology to design, validate and evaluate a multiplex panel for detection of B-ALL fusion- transcripts. Fifty-one B-ALL fusion transcripts reported in children in the literature were included in the design of the nanoString panel. Twenty-six fusion transcripts were validated using 64 positive control samples and 74 negative control samples with 100% sensitivity and 99% in comparison to RT-PCR. Our results support a potential role of nanoString as a robust and cost-effective technique that could be used in the detection of fusion transcripts and implemented in diagnostic algorithm of B-ALL. Please refer to more detailed description under each file inside the folders.