IDENTIFIKASI KERAGAMAN GEN MxSEBAGAI PENANDA GENETIK RESISTEN VIRUS AVIAN INFLUENZAPADA AYAM KAMPUNG DI KABUPATEN KAMPAR
| Main Author: | Ridho Rizkillah Putra, |
|---|---|
| Format: | Thesis NonPeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
, 2015
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| Subjects: | |
| Online Access: |
http://repository.uin-suska.ac.id/5852/1/fm.pdf http://repository.uin-suska.ac.id/5852/2/BAB%20I.pdf http://repository.uin-suska.ac.id/5852/3/BAB%20II.pdf http://repository.uin-suska.ac.id/5852/4/BAB%20III.pdf http://repository.uin-suska.ac.id/5852/5/BAB%20IV.pdf http://repository.uin-suska.ac.id/5852/6/em.pdf http://repository.uin-suska.ac.id/5852/ |
Daftar Isi:
- The Mx gene that associated with the avian influenza virus resistance could be used as genetic marker of the avian influenza virus resistance. The purpose of this research was to know the polymorphism of Mx gene of kampong chicken in Kampar regency using the Polymerase Chain Reaction – Restriction Fragment Length Polymorphism (PCR-RFLP) method. The research used 30 samples of kampong chicken blood from three different villages in Kampar regency i.e. Rumbio Jaya in Alam Panjang sub district, Salo in Salo sub district, and Simpang Kubu in Kampar sub district. The data of Mx gen genotype analyzed by the genotype and the allele frequencies approach and the heterozygosity value estimation. The amplification of the Mx gene resulted DNA fragment as long as 299 bp. The restriction of the fragment using Hpy81 generated three genotypes namely AA (shown by single band of 299 bp DNA), AG (shown by three bands of 299, 200 and 99 bp DNA) and GG (shown by two bands of 200 and 99 bp DNA). The frequency of the AA genotype higher than AG and GG genotype in Alam Panjang and Salo. The frequency of A allele higher than G allele in all villages i.e. Alam Panjang 90%, Salo 85% and Simpang Kubu 60%. The value of heterozygosity observation was highest in Simpang Kubu and was lowest in Alam Panjang. The conclusion was Mx│Hpy81 gene of kampong chicken in Kampar regency was polymorphic with the finding of three genotypes (AA, AG and GG) and two alleles (A and G). Keyword : kampong chicken, Mx│Hpy81 gene, PCR-RFLP, polymorphism