OPTIMASI MEDIA TANAM TERHADAP INDUKSI KALUS DARI PETIOL PASAK BUMI (Eurycoma longifoliaJack) SECARA IN VITRO
| Main Author: | Maisupratina, |
|---|---|
| Format: | Thesis NonPeerReviewed Book |
| Bahasa: | eng |
| Terbitan: |
, 2015
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| Subjects: | |
| Online Access: |
http://repository.uin-suska.ac.id/5757/1/FM.pdf http://repository.uin-suska.ac.id/5757/2/BAB%201.pdf http://repository.uin-suska.ac.id/5757/3/BAB%202.pdf http://repository.uin-suska.ac.id/5757/4/BAB%203.pdf http://repository.uin-suska.ac.id/5757/5/BAB%204.pdf http://repository.uin-suska.ac.id/5757/6/BAB%205.pdf http://repository.uin-suska.ac.id/5757/7/EM.pdf http://repository.uin-suska.ac.id/5757/ |
Daftar Isi:
- Eurycoma longifolia Jack is a medicinal plant that contains the active chemical ingredients derived from the results of secondary metabolites when have useful biological activity for medicine. Because of strong consumer demand of Eurycoma longifolia Jack may treat this species, hance, one of the most efficient alternative in dealing with this problem is through tissue culture propagation. The purpose of this study was to determine the best optimization 1⁄2MS and MS0 basic media with the addition of PGR. ZPT was used in the form of 2.4-D whit four concentrations (0,1,2,3 and 4 ppm) and kinetin with three concentrations (0,1,2 ppm). Explants were taken from the petiole. This study used descriptive qualitative and quantitative methods. Variables measured included time emerge callus, callus color, browning percentage, the percentage of contamination, percentage of grows, and texture callus. The results of this study on media MS0 showed that there were four treatments able to induce callus namely, 2.4-D 0 ppm + Kinetin 1 ppm, 2.4-D 1 ppm + 2 ppm Kinetin, 2.4-D 3 ppm + Kinetin 1ppm, and 2.4-D 4 ppm + 1 ppm Kinetin with less compact texture, meanwhile the rest treatments were browning and dormancy. The fastest time in inducing callus was found in the fourth week and the longest time in the sixth week, With high contamination level. 1⁄2 MS media did not able to induce callus 1⁄2MS but experienced browning . Keywords: Eurycoma longifolia Jack, Kinetin, 2,4 D, callus.