Validation gas chromatography-fid method for analysis of ethanol content in vinegar
| Main Authors: | Pulungan, Indah Noviyanti Ruhmana, Kartosentono, Sugijanto, Prawita, Amirudin |
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| Format: | Article info application/pdf Journal |
| Bahasa: | eng |
| Terbitan: |
Universitas Airlangga
, 2018
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| Subjects: | |
| Online Access: |
https://e-journal.unair.ac.id/JHPR/article/view/10508 https://e-journal.unair.ac.id/JHPR/article/view/10508/5803 |
Daftar Isi:
- Production process of vinegar could be synthesized by chemical or microbiological. In Islam, vinegar must not be consumed if contains ethanol ≥ 1%, because it is categorized into khamr. The maximum concentration of vinegar fermentation is 1,0 %. This research was to analyze the alcohol compund that consist in vinegar. The validation method that used in this study are: selectivity, linearity, range, precision, accuracy, LOD and LOQ. The analysis instrument was gas chromatography Agilent 6890 Series GC System, with a Flame Ionization Detector (FID) and a column HP-5 (5% Phenyl 95% Methyl Siloxane), and optimal conditions were obtained by using temperature of inlet and detector of 250°C whilst the temperature of oven programed initially at 40°C, hold for 5 minutes, and raised to 5°C/min to 200°C. were used in this study. For split ratio was achieved at 50:1 and the injection volume was 1.0μl. The methods were proven selective and demonstrated linearity, precision and accuracy with r = 0.9995; 5,63% and 101,25%, respectively. On the other hand, the methods were proven that the sample contains alcohol. Finally, the result of quantitative ethanol contents analysis in vinegar from Saudi Arabia is 2,28.10-2 % v/v; and vinegar brand “x” from market in Surabaya is 1,17.10-2 v/v.