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UJI BIOKIMIA UNTUK IDENTIFIKASI MEKANISME RESISTENSI GANDA VEKTOR MALARIA TERHADAP INSEKTISIDA DI JAWA TIMUR

Main Authors: -, Widiarti; B2P2VRP Salatiga, Boewono, Damar Tri; B2P2VRP Salatiga, -, Mujiono; B2P2VRP Salatiga
Format: application/pdf eJournal
Bahasa: ind
Terbitan: Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (B2P2VRP) Salatiga , 2011
Online Access: http://ejournal.litbang.depkes.go.id/index.php/vk/article/view/3
ctrlnum article-3
fullrecord <?xml version="1.0"?> <dc schemaLocation="http://www.openarchives.org/OAI/2.0/oai_dc/ http://www.openarchives.org/OAI/2.0/oai_dc.xsd"><title lang="id-ID">UJI BIOKIMIA UNTUK IDENTIFIKASI MEKANISME RESISTENSI GANDA VEKTOR MALARIA TERHADAP INSEKTISIDA DI JAWA TIMUR</title><creator>-, Widiarti; B2P2VRP Salatiga</creator><creator>Boewono, Damar Tri; B2P2VRP Salatiga</creator><creator>-, Mujiono; B2P2VRP Salatiga</creator><subject lang="id-ID"/><subject lang="id-ID"/><subject lang="id-ID"/><description lang="id-ID">Resistance is inherited and has proved to be the biggest single barrier to successful chemical control of insect vectors. The continuity of along time period insecticide usage can produce mosquitoes resistance. Resistance to insecticide as a results from three main mechanism : 1) insecticide penetration is reduce, 2) the insecticides is more efficiently metabolized by esterases, mixed function oxidases, or glutathione transferase enzyme and, 3) the target of the insecticide is modified (insensitive acetylcholinesterase). The objectives of this study was to determine the potency of malaria vector from East Java Province to be resistant to organophosphate, carbamate and pyrethroid insecticides. The research methods used were biochemical assays (microplate assays) for elevated esterase and insensitive acetylcholinesterase. The esterase activity and insensitive acetylcholinesterase were measured at 450 nm and 405 nm with a Dytech Elisa plate reader. Biochemical assays indicated that susceptibility (resistant or tolerance), of the malaria vector collected from East Java Province natural population against insecticide were mostly decreased, although there were different level and mechanism occurs. Microplate enzymatic assay on individual Anopheles sundaicus collected from Banyuwangi, Pacitan, Jember, Malang Regency revealed that 31,25 %, 12,5 %, 45,2 % and 15,25 % population were resistant respectively due to elevated esterase activity mechanism. Base on the susceptibility test which was held using WHO method (as a cross-check) An. sundaicus from Teleng village, Pacitan Regency was proven to have a double resistance agains Malathion 0,5 % (cause mortality 28 %) and Bendiocarb 0,1 % (cause mortality 66 %). The percentage resistance of Anopheles aconitus population collected from Pacitan and Trenggalek Regency were 35,42 % and 29,17 % population respectively due to elevated esterase activity mechanism. There was no evidence of an altered acetylcholinesterase (insensitive acetylcholinesterase) mechanism of the malaria vector population in East Java.</description><publisher lang="en-US">Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (B2P2VRP) Salatiga</publisher><contributor lang="id-ID"/><date>2011-12-02</date><type>Other:</type><type>Other:</type><type>File:application/pdf</type><identifier>http://ejournal.litbang.depkes.go.id/index.php/vk/article/view/3</identifier><source lang="id-ID">Vektora; Vol 1, No 1 Jun (2009); 23-33</source><source lang="en-US">Vektora : Jurnal Vektor dan Reservoir Penyakit; Vol 1, No 1 Jun (2009); 23-33</source><language>ind</language><coverage lang="id-ID"/><coverage lang="id-ID"/><coverage lang="id-ID"/><rights>The Authors submitting a manuscript do so on the understanding that if accepted for publication, copyright of the article shall be assigned to VEKTORA and Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (Institute of Vector and Reservoir Control Research and Development)&#xA0;as publisher of the journal. Copyright encompasses exclusive rights to reproduce and deliver the article in all form and media, including reprints, photographs, microfilms and any other similar reproductions, as well as translations. The reproduction of any part of this journal, its storage in databases and its transmission by any form or media, such as electronic, electrostatic and mechanical copies, photocopies, recordings, magnetic media, etc. , will be allowed only with a written permission from VEKTORA (Journal of Vector Borne and Reservoir Diseases) and Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (Institute of Vector and Reservoir Control Research and Development). VEKTORA (Journal of Vector Borne and Reservoir Diseases)&#xA0;and Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (Institute of Vector and Reservoir Control Research and Development), the Editors and the Advisory International Editorial Board make every effort to ensure that no wrong or misleading data, opinions or statements be published in the journal.</rights><recordID>article-3</recordID></dc>
language ind
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author -, Widiarti; B2P2VRP Salatiga
Boewono, Damar Tri; B2P2VRP Salatiga
-, Mujiono; B2P2VRP Salatiga
title UJI BIOKIMIA UNTUK IDENTIFIKASI MEKANISME RESISTENSI GANDA VEKTOR MALARIA TERHADAP INSEKTISIDA DI JAWA TIMUR
publisher Balai Besar Penelitian dan Pengembangan Vektor dan Reservoir Penyakit (B2P2VRP) Salatiga
publishDate 2011
url http://ejournal.litbang.depkes.go.id/index.php/vk/article/view/3
contents Resistance is inherited and has proved to be the biggest single barrier to successful chemical control of insect vectors. The continuity of along time period insecticide usage can produce mosquitoes resistance. Resistance to insecticide as a results from three main mechanism : 1) insecticide penetration is reduce, 2) the insecticides is more efficiently metabolized by esterases, mixed function oxidases, or glutathione transferase enzyme and, 3) the target of the insecticide is modified (insensitive acetylcholinesterase). The objectives of this study was to determine the potency of malaria vector from East Java Province to be resistant to organophosphate, carbamate and pyrethroid insecticides. The research methods used were biochemical assays (microplate assays) for elevated esterase and insensitive acetylcholinesterase. The esterase activity and insensitive acetylcholinesterase were measured at 450 nm and 405 nm with a Dytech Elisa plate reader. Biochemical assays indicated that susceptibility (resistant or tolerance), of the malaria vector collected from East Java Province natural population against insecticide were mostly decreased, although there were different level and mechanism occurs. Microplate enzymatic assay on individual Anopheles sundaicus collected from Banyuwangi, Pacitan, Jember, Malang Regency revealed that 31,25 %, 12,5 %, 45,2 % and 15,25 % population were resistant respectively due to elevated esterase activity mechanism. Base on the susceptibility test which was held using WHO method (as a cross-check) An. sundaicus from Teleng village, Pacitan Regency was proven to have a double resistance agains Malathion 0,5 % (cause mortality 28 %) and Bendiocarb 0,1 % (cause mortality 66 %). The percentage resistance of Anopheles aconitus population collected from Pacitan and Trenggalek Regency were 35,42 % and 29,17 % population respectively due to elevated esterase activity mechanism. There was no evidence of an altered acetylcholinesterase (insensitive acetylcholinesterase) mechanism of the malaria vector population in East Java.
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