Physicochemical Properties and Antioxidant Activities of Crude Fucoidan extracted from Sargassum cinereum
| Main Authors: | Nurhidayati, Liliek, Fitriani, Yashinta, Abdillah, Syamsudin, Mumpuni, Esti, Rafi, Mohamad |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Fakultas Farmasi Universitas Pancasila
, 2020
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| Online Access: |
http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/823 http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/823/522 |
Daftar Isi:
- Brown seaweed has been known as a fucoidan source that has various biological activities. Fucoidan could be obtained by various extraction methods. This study aims to extract, characterize and to evaluate the antioxidant activity of crude fucoidan extracted from Sargassum cinereum. Extraction was carried out by refluxing at 100oC for 4 and 5 hours and then precipitated with ethanol. The crude fucoidan was brown powder. The yield with 4 hours extraction time was 2.78% which did not differ significantly from the 5 hours extraction time. Phytochemical screening of crude extract showed that beside fucoidan there were flavonoids, saponins, steroids, triterpenoids, and tannins. The extraction with duration of 4 hours and 5 hours had sulfate content of 16.31% and 11.22%, total carbohydrate content were 22.94% and 22.85%, and in the FTIR spectrum showed OH absorption bands of carbohydrates, fucose, sulfates and uronic acid. Testing of antioxidant activity using the DPPH (1,1-diphenyl-2-picrylhyrazyl) free radical inhibition method produced IC50 values of 721.9 μg/ml and 749.9 μg/ml respectively and it was concluded that crude fucoidan of Sargassum cinereum had weak antioxidant activity.
- Rumput laut coklat telah dikenal sebagai sumber fukoidan yang memiliki berbagai aktivitas biologi. Fukoidan bisa diperoleh dengan berbagai cara ekstraksi. Penelitian ini bertujuan untuk mengekstraksi, mengkarakterisasi dan menguji aktivitas antioksidan crude fukoidan hasil ekstraksi dari Sargassum cinereum. Ekstraksi crude fukoidan dilakukan dengan merefluks pada suhu 100oC dengan lama waktu 4 dan 5 jam kemudian diendapkan dengan etanol. Hasil ekstraksi berupa serbuk coklat kehitaman. Rendemen dengan lama ekstraksi 4 jam sebesar 2,78% yang tidak berbeda bermakna dengan lama ekstraksi 5 jam. Penapisan fitokimia terhadap crude fukoidan menunjukkan bahwa selain fukoidan, terdapat senyawa golongan flavonoid, saponin, steroid, triterpenoid, dan tannin. Hasil ekstraksi 4 jam dan 5 jam memiliki kadar sulfat sebesar 16,31% dan 11,22%, kadar karbohidrat total 22,94 % dan 22,85%, dan pada spektrum FTIR menunjukkan pita serapan OH dari karbohidrat, fukosa, sulfat, dan asam uronat. Pengujian aktivitas antioksidan menggunakan metode peredaman radikal bebas DPPH (1,1- difenil 2-pikrihidrazil) menghasilkan nilai IC50 untuk ekstrak 4 jam dan 5 jam sebesar 721,9 μg/ml dan 749,9 μg/ml. Dapat disimpulkan bahwa crude fukoidan Sargassum cinereum memiliki aktivitas antioksidan yang lemah.