Isolation and Identification of Phenolic Compounds from Macaranga hispida Blume Mull.Arg Leaves
| Main Authors: | Megawati, Megawati, Fajriah, Sofa, Widyawati, Galuh, Darmawan, Akhmad |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Fakultas Farmasi Universitas Pancasila
, 2020
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| Online Access: |
http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/722 http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/722/543 |
Daftar Isi:
- Continuation of research on Macaranga plant has been conducted. In this study, the totalphenol content (TPC) of Macaranga hispida Blume Mull. Arg leaves extracts were investigated. Gallicacid (1) and methyl gallate (2) have been isolated from the ethyl acetate fraction which highest phenoliccontent (8.41 mg GAE/g). Serial techniques of chromatographic and spectroscopic methods, namelyextraction, fractionation, purifi cation, FTIR, LCMS, and FT-NMR were used to isolate and identify theisolates. Cytotoxicity of compound 1 and 2 against P-388 cell line showed that compound 2 has higheranticancer activity compared to compound 1 with IC50 value of 75.53 μg/mL.
- Kelanjutan penelitian pada tanaman Macaranga telah dilakukan. Pada penelitian ini dilakukankandungan fenol total dari ekstrak daun Macaranga hispida Blume Mull.Arg. Asam galat (1) dan metilgalat (2) telah diisolasi dari fraksi etil asetat yang memiliki kandungan fenolik tertinggi (8,41 mg GAE/g). Serangkaian teknik kromatografi dan spektroskopi yaitu ekstraksi, fraksinasi, pemurnian, FTIR,LCMS, dan FT-NMR digunakan untuk mengisolasi dan identifi kasi isolat. Sitotoksisitas senyawa 1 dan2 terhadap sel murine leukimia P-388 menunjukkan bahwa senyawa 2 memiliki aktivitas antikankeryang lebih tinggi dibandingkan dengan senyawa 1 dengan nilai IC50 75,53 μg/mL.