The In Vitro and In Silico Study of n’-benzoylisonicotinohydrazide as Antituberculosis Candidate
Main Authors: | Ruswanto, Ruswanto, Rahayuningsih, Nur, Hidayati, Nur Laeli Dwi, Nuryani, Ginna Sri, Mardianingrum, Richa |
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Format: | Article info application/pdf eJournal |
Bahasa: | eng |
Terbitan: |
Fakultas Farmasi Universitas Pancasila
, 2019
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Online Access: |
http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/703 http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/703/503 |
Daftar Isi:
- There have been in silico and in vitro studies of N-Benzoylisonicotinohydrazide derivatives as antituberculosis candidates. The aims of this research were determined that the N'-benzoylisonicotinohydrazide derivatives could inhibit the activity of gram-positive, gram-negative and Mycobacterium tuberculosis, as well as having good interactions with Enoyl-Acyl Carrier Protein Reductase from Mycobacterium Tuberculosis. From the in vitro test it was found that the N'-benzoylisonicotinohydrazide compound had a Minimum Inhibitor Concentration (MIC) of 0.33 μg / ml against the Basillus subtilis, while the Minimum Inhibitor Concentration (MIC) of N '- (2-chlorobenzoyl) isonicotinohydrazide against Mycobacterium tuberculosis (H37Rv) was 3.125 μg / ml. From the in silico study, it was found that the binding affinity value between N '- (2-chlorobenzoyl) isonicotinohydrazide enzyme Enoyl-Acyl Carrier Protein Reductase (2X23) had the smallest binding affinity so that it could be predicted that it had a stable interaction than other compounds so that the N '- (2-chlorobenzoyl) isonicotinohydrazide could be used as a more potent antituberculosis candidate.
- Telah dilakukannya penelitian tentang studi in vitro dan in silico senyawa turunan N-Benzoylisonicotinohydrazide. Penelitian ini bertujuan untuk mengetahui bahwa senyawa turunan N’-benzoylisonicotinohydrazide dapat menghambat aktivitas bakteri gram positif, gram negatif dan Mycobacterium tuberculosis, serta mempunyai interaksi yang baik dengan Enoyl-Acyl Carrier Protein Reductase dari Mycobacterium Tuberculosis. Dari uji in vitro dihasilkan bahwa senyawa N’-benzoylisonicotinohydrazide memiliki Minimum Inhibitor Concentration (MIC) sebesar 0,33 μg/ml terhadap bakteri Basillus subtilis, sedangkan besarnya Minimum Inhibitor Concentration (MIC) senyawa N’-(2-chlorobenzoyl)isonicotinohydrazide terhadap Mycobacterium tuberkulosis (H37Rv) adalah sebesar 3,125 μg/ml. Dari studi in silico didapat bahwa nilai binding affinity antara senyawa N’-(2-chlorobenzoyl)isonicotinohydrazide enzim Enoyl-Acyl Carrier Protein Reductase (2X23) mempunyai binding affinity yang paling kecil sehingga dapat diprediksi bahwa senyawa tersebut mempunyai interaksi yang stabil dan paling baik disbanding senyawa-senyawa lainnya sehingga senyawa N’-(2-chlorobenzoyl)isonicotinohydrazide dapat digunakan sebagai kandidat antituberkulosis yang lebih poten.