Determination of Quality Parameters and Antioxidant Activity of Cincau Hijau Leaves (Cyclea barbata L.Miers.)
| Main Authors: | Gangga, Erlindha, Purwati, Rani, Farida, Yunahara, Kartiningsih, Kartiningsih |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Fakultas Farmasi Universitas Pancasila
, 2017
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| Online Access: |
http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/525 http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/525/357 |
Daftar Isi:
- Cincau Hijau leaves (Cyclea barbata L.Miers ) has been used empirically as a medicinal plant because it contains many potential compounds such as flavonoids. Cincau Hijau leaves has been known as antioxidant activity. Determination of pharmacognosy parameters and phytochemical screening of dry leaves and extracts have been carried out as well as determination of total fl avonoids content. Fresh leaves were extracts with water and Dry leaves were macerated with 96% ethanol and were then concentrated by rotavapor to obtain viscous extracts. Free radical scavenging activity of the extracts was evaluated using DPPH method. Afterwards, determination of specifi c and non-specifi c parameters were performed. Results of phytochemical screening of powder and 50% and 96% ethanol extract showed that tall the tested samples contained alkaloid, fl avonoids, saponins, tannins, steroids/ triterpenoids, coumarin. The examination of specifi c parameter showed that the extract has a thick consistency, tawny color, bitter taste, characteristic odor. In addition, water-soluble compound and 96% ethanol extract are 46.64 and 62.13% respectively whereas ethanol-soluble compounds are 39.22 and 74.72%, respectively. While the results of nonspecifi c parameters of 50% and 96 % ethanol extract displayed total ash content of 9.69 and 9.49%, respectively, acid insoluble ash content of 0.30 and 0.16%, respectively, content of water soluble ash of 9.17 and 4.30%, respectively, loss on drying of 9.35 and 8.9%, respectively, water content of 8.45 and 7.25%, respectively. Based on heavy metal contamination, Pb concentration in 50 and 96% ethanol extract are 0.0227 and 0.0333 mg/kg, respectively whereas Cd concentration are 0.1206 and 0.0022 mg/kg, respectively and total number of CFU of 4,22 x 103 and 2,30 x 103 colonies/g, respectively while molds and yeasts number of colony of 0,48 x 102 and 8,88 x 102 colonies/g, respectively. Moreover, the total flavonoid was 0,19 %. Result of DPPH inhibition test showed that IC50 96 % ethanol extract are 83,280 ppm and water extracts are 102,01 ppm
- Cyclea barbata dikenal masyarakat sebagai obat tradisional dan dapat diolah menjadi minuman penyegar. Manfaat lainnya yaitu sebagai antioksidan yang dapat meredam radikal bebas. Penelitian ini bertujuan untuk menguji aktivitas antioksidan dengan metode peredaman radikal bebas DPPH dan menetapkan mutu ekstrak, serta kadar flavonoid total daun cincau hijau. Hasil penapisan fitokimia simplisia, ekstrak, dan air perasan menunjukkan adanya senyawa alkaloid, flavonoid, saponin, tanin, steroid, triterpenoid, dan kumarin. Hasil penetapan mutu ekstrak etanol 96% menunjukkan ekstrak dengan konsistensi kental, berwarna hijau kehitaman dan berbau khas spesifik. Kadar sari larut air 74,72%, kadar sari larut etanol 62,13%, susut pengeringan 10,86%, kadar air 9,26%, kadar abu total 9,49%, kadar abu tidak larut asam 0,16%, sisa pelarut etanol 0,46%, cemaran logam Pb 0,03 mg/kg dan Cd 0,02 mg/kg, cemaran mikroba dengan angka lempeng total 2,30x103 koloni/g dan angkakapang khamir 8,88x102 koloni/g. Hasil penetapan kadar flavonoid total menunjukkan kadar flavonoid sebesar 8,30%. Dari hasil penelitian dapat disimpulkan bahwa ekstrak etanol 96% daun cincau hijau memiliki mutu ekstrak yang baik dan aktivitas antioksidan dengan metoda peredaman radikal bebas menggunakan DPPH adalah pada ekstrak kental menunjukkan nilai IC50 sebesar 83,28 bpj, dan air perasan dengan IC50 102,01 bpj.