Nanoparticle Formulation of Pineapple Stem Extract (Ananas Comosus (L).(Merr) as Antimicrobial Agent
| Main Authors: | Flareyanti, Dian Eleveny Martha, Fahleni, Fahleni, Rahmat, Deni |
|---|---|
| Format: | Article info application/pdf eJournal |
| Bahasa: | eng |
| Terbitan: |
Fakultas Farmasi Universitas Pancasila
, 2017
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| Online Access: |
http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/513 http://jifi.farmasi.univpancasila.ac.id/index.php/jifi/article/view/513/325 |
Daftar Isi:
- Pineapple stem contains bromelain enzyme with highest concentration compared to the other parts. The aim of the research was to formulate an anti dandruff creambath containing nanoparticles of pineapple stem which was physically and chemically stable and could inhibit the growth of Pityrosporum ovale. The pineapple stem extract was then formulated into nanoparticles prepared by cross-linking method using chitosan. The resulting nanoparticles were dried using freeze drying. The dried powder of extract was used to determine the minimum inhibitory concentration (MIC) against Pityrosporum ovale. The zone of inhibition of extract and nanoparticles were measured at the MIC. The extracts were then formulated into anti dandruff creambath. The creambath in the concentration of 3% (MIC) whereas the nanoparticles were formulated in the consentration of 1, 2, 3 fold MIC. Each formula was evaluated for its physical characteristics including organoleptics, homogenity, viscosity and flow properties, spread ability and for its chemical characteristic pH value. The stability was performed for one month at temperature of 25 oC and 40 oC. The results showed that the nanoparticles have particle size of 860.03 nm and zeta potensial of 18.63 mV. The best formula was formula IV with inhibitory zone of 17 mm, and had stable organoleptic properties, homogenity, viscosity and flow properties, and pH value, as well as capaability of spreading with diameter of 4817.53 mm. Accordingly the nanoparticlesin creambath could increase the antimicrobial activity.
- Bonggol nanas (Ananas comosus (L).(Merr) mengandung enzim bromelin lebih tinggi dibandingkan bagian buahnya. Tujuan penelitian ini adalah untuk memformulasi creambath antiketombe dari nanopartikel ekstrak bonggol nanas yang stabil secara fi sika, kimia dan dapat menghambatpertumbuhan Pityrosporum ovale. Ekstrak bonggol nanas dibuat dalam bentuk nanopartikel dengan menggunakan kitosan dengan metode taut silang yang kemudian dikeringkan dengan metode freeze drying. Serbuk kering ekstrak ditentukan konsentrasi hambat minimumnya terhadap Pityrosporum ovale. Dibuat satu formula yang mengandung ekstrak bonggol nanas dengan konsentrasi 3% dan tiga formula yang mengandung nanopartikel ekstrak bonggol nanas dengan konsentrasi 1xKHM, 2xKHMdan 3xKHM. Tiap sediaan dilakukan uji mutu fisik antara lain organoleptik, homogenitas, viskositas dan sifat alir, uji daya sebar, serta mutu kimia yaitu pH dan diuji stabilitasnya selama satu bulan padasuhu 25 oC dan 40 oC. Hasil penelitian menunjukan bahwa nanopartikel ekstrak bonggol nanas memiliki rata-rata ukuran partikel 860,03 nm dan zeta potensial 18,63 mV. Creambath terbaik adalah sediaan yang mengandung nanoekstrak 3XKHM dengan DDH 17 mm, memiliki organoleptik, homogenitas, viskositas dan sifat alir, dan pH yang stabil, serta memiliki kemampuan menyebar 4817,53 mm2. Dengan demikian nanopartikel dalam creambath bisa meningkatkan aktivitas antimikroba dari bonggol nanas.